摘要
作者应用亲和色谱,从Ⅰ型单疱病毒(HSV 1)免疫山羊的淋巴细胞透析物,分离HSV-1特异转移因子(TF_(HSV-1))活性组分,再以^(51)Cr标记的白细胞粘附抑制试验(^(51)Cr-LAIT)和高效液相疏水色谱(HI-HPLC)鉴定该组分的生物活性和纯度。结果显示,亲和色谱分离的TF_(HSV-1)组分,能诱导正常小鼠脾细胞产生对HSV-1特异抗原的粘附抑制效应,具有TF的抗原特异活性;该组分经HI-HPLC分析,在色谱图上仅呈现两个分离峰.上述结果表明,已从TF_(HSV-1)粗提物中分离到部分纯化的TF_(HSV-1)活性组分.亲和色谱是分离TF的有效方法。
The active components of herpes simplex virus type 1 (HSV-1) specific transfer factor (TFHSV-1) were separated from dialysates of goats lymphocytes immunized with the corresponding virus using the affinity chromatographic technique. The biological activity and purity of the transfer factor components were then evaluated by 51Cr labeled leukocyte adherence inhibition (51Cr LAI) assay and hydrophobic interaction high-performance liquid chromatography (HI-HPLC). It was thus shown that the separated TF components induced obviously the splenic cells of normal mice to produce LAI effect on HSV-1 antigen, but not on control antigens, indicating that the separated components has the antigen-specific activity of TFHSV-1. Analyzed by HI-HPLC, the active components appeared only two peaks in the chromatogram. It seems, therefore, that partially purified transfer factor active components were obtained from the dialysates, and the affinity chromatographic method on HSV-1 antigen absorbent employed here is well suited for the isolation of complex dialysates containing TF.
出处
《第四军医大学学报》
1992年第2期106-109,共4页
Journal of the Fourth Military Medical University
关键词
转移因子
人疱疹病毒
色谱法
transfer factor
human herpes virus
chroma tography
affinity
