摘要
本实验以裸鼠人肝细胞癌动物模型,应用磺化氯铝酞菁(ALSPC)为光敏剂,观察了ALSPC光敏治疗后肿瘤组织的形态学改变。治疗后光镜下见肿瘤细胞明显变性、解离,胞体呈气球样变,胞核固缩,同时毛细血管内皮损伤,瘤组织弥漫性出血。电镜下见部分粗面内质网扩张,线粒体肿胀,核周间隙扩大,最终核染色质凝集,核膜胞膜破裂,细胞死亡。内皮细胞也出现类似改变。提示ALSPC对接种于动物体内的人肝细胞癌有明显的杀伤作用,是一种有效的光敏剂,细胞的膜系统可能是主要作用靶位。
The morphologic changes of human hepatocaranoma transplanted in nude mice afler photodynamic therapy (PDT) with sulfonated aluminum phthalocyanine (ALSPQ were studied The cancer cells after PDT with ALSPC showad vacuolar degeneration and separation from each other. The endothelium of blood vessel was damaged and hemorrhage occured Under electron microscope the endoplasmic reticulum enlarged, mitochondria swelled, perinudear space dilated, and chromosome aggregated and condensed. The membrane of nuclei and cells broke off and tumor cells died at last The endothdial cells had the same changes mentioned above. This result suggests that ALSPC has photodynamic effect on the human hepatocellular carcinoma tranaplanted in nude mice. And the membrane system of cells is probably the main target.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1992年第3期229-232,共4页
Academic Journal of Second Military Medical University
关键词
酞染料
药物疗法
肝肿瘤
细胞癌
phthaldn dyes
photochemotherapy
hepatoma
ultrastructure
animal
nude mice
