摘要
目的 研究Losartan和人参对缺血再灌注心肌细胞凋亡的影响 ,比较Losartan和人参对缺血再灌注心肌细胞损伤的保护作用。方法 结扎Wistar大鼠左冠状动脉前降支 ,建立大鼠缺血再灌注动物模型 ,采用末端标记原位细胞凋亡法检测心肌细胞凋亡 ,并利用光学显微镜进行细胞计数。结果 单纯缺血 再灌注组心肌细胞凋亡数较假手术组明显增多(37 5± 9 2 2 /视野vs 0 18± 0 0 91/视野 ,P <0 0 5 ) ,Losartan组和人参组心肌细胞凋亡数分别为 6 5 0± 3 5 9/视野和 8 74± 3 5 1/视野 ,较单纯缺血 再灌注组明显减少 (P <0 0 5 ) ,Losartan和人参两组间无明显区别 (P >0 0 5 )。结论 缺血再灌注可引起明显的心肌细胞凋亡 ,Losartan和人参对缺血再灌注心肌损伤具有相似的保护作用 ,均可明显减少缺血再灌注心肌细胞凋亡。
? Objective To compare theprotectiveeffect of Ginseng and Losartan on cardiomyocyte apoptosisin the experimentalcardiomyocyteischemia/reperfusion of rats. Method The left anterior descending branch of the coronary artery was ligated to institute the ischemia/ reperfusion heart model in Wistar rats. The apoptotic cardiomyocytes wereshown by in situ end labeling method and their number was counted under optical microscopy. Result The result showed that the number of apoptotic cardiomyocytes per visual field in the ischemia/ reperfusion group (37.5±9.22) was much larger than that in the sham operation group (0.18±0.091) (P<0.05). The number of apoptotic cardiomyocytes per visual field in the Ginseng-treated group (8.74±3.51) and Losartan-treated group (6.50±3.59) was much smaller than that in the ischemia/reperfusion group (P<0.05), but there was no signigficant difference between the Ginseng treated group and Losartan-treated group (P>0.05). Conclusion Ginseng and Losartan have the same anti-apoptotic effect on cardiomyocytes in ischemia/reperfusion. 〔
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2002年第4期451-453,516,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
湖北省自然科学基金资助(No.2000J050)
