摘要
目的 探讨白细胞介素 - 17(IL - 17)在狼疮性肾炎患者 (LN)外周血单个核细胞 (PBMC)表达和分泌及其与LN疾病活动性的关系。方法 用酶联免疫吸附法 (ELISA)检测LN活动期患者血浆及PBMC体外自发培养或刺激培养的上清液中IL - 17蛋白水平 ,流式细胞仪免疫荧光分析法检测PBMC表达IL - 17的水平 ;用逆转录 -聚合酶联反应 (RT -PCR)法测定LN活动期患者PBMC体外自发培养和刺激培养后的IL - 17mRNA的表达水平。结果 LN活动期患者血浆检测不出IL - 17(<5ng/L) ;PBMC自发培养和PHA刺激培养下 ,LN活动期、LN静止期和正常对照 ,三组均检测不出IL - 17;而PBMC在CD3mAb +CD2 8mAb和PMA +ionomycin刺激培养下 ,较自发培养和PHA刺激培养 ,上述 3组IL - 17水平均明显升高 (P <0 .0 1) ,LN活动期PBMC表达、分泌IL - 17水平与SLE疾病活动指数 (SLEDAI)呈明显正相关。结论 LN活动期患者PBMC表达、分泌IL -17水平存在明显异常 。
Objective\ To know expression of IL-17 in the peripheral blood cells (PBMC) of LN and relationship of IL-17 level with SLE disease activity index(SLEDAI).Methods\ Plasma and PBMC culture supernatants IL-17 was measured with ELISA,and IL-17 in PBMC by immunofluorescent staining using specific antibody and flow cytometric analyses.IL-17 mRNA was detected by RT-PCR techniques.Results\ The completely stimulated(CD3mAb+CD28mAb or PMA+lonomycin)culture supernatants IL-17,IL-17 or its mRNA level in the complete stimulated PBMC,but not plasma IL-17 level,were significantly elevated in active LN patients as compared to the normal control.The culture supernatants IL-17 level and the IL-17 mRNA level in the PBMC were strongly correlated with SLEDAI.Conclsions\ IL-17 protein or mRNA expression in the PBMC and IL-17 level in the completely stimulated culture supernatants of active LN patients are abnormally elevated and its increased level can be a significant indicator for LNactivity degree.
出处
《中国实用内科杂志》
CAS
CSCD
北大核心
2002年第12期735-736,共2页
Chinese Journal of Practical Internal Medicine
