摘要
目的 观察γ 射线诱导p5 3基因突变的白血病细胞株Jurkat细胞凋亡的时间和剂量效应 ,以探求辐射诱发肿瘤细胞凋亡通路的分子机制。方法 分别以 5、10、2 0Gyγ 射线照射Jurkat细胞 ,于照射后继续培养 6、12、2 4、3 6、48h ,分别收获细胞。流式细胞术 (FCM )定量观察细胞凋亡的变化。采用WesternBlot法检测Jurkat细胞株p5 3基因蛋白的表达。结果 细胞受照射后 ,在一定的时间范围内 ,凋亡细胞的比例随作用时间和照射剂量的增加而增加 ,显示出较好的量效和时效关系。本实验所用的Jurkat细胞株是p5 3基因突变的细胞株 ,p5 3基因蛋白不表达。 结论 确立了γ 射线诱导Jurkat细胞凋亡时间和剂量效应关系 ,而导致凋亡的机制独立于肿瘤抑制基因 p5 3 ,这为探讨新的凋亡通路机制奠定了基础。
Objective To study the time and dose kinetics of cell apoptosis induced by γ radiation in the human leukemia T cell line Jurkat and to look for the mechanisms of radiation induced apoptosis of human tumors. Methods Jurkat cells were exposed to γ radiation of 5,10,20 Gy. The radiated cells were incubated for 6,12,24,36,48h after radiation. The quantification of the apoptosis cells was performed by flow cytometry(FCM). The expression of p53 protein was examined by Western blot.Results After radiation, the proportion of apoptotic cells increased with the increment of the radiation doses and the incubation time after irradiation in a given range. The expression of p53 was absent in Jurkat cell before and after radiation. Conclusion The windows of the time and dose of cell apoptosis induced by γ radiation in Jurkat are established.Understanding of ionizing radiation-induced apoptosis being independent of p53 paves the way for looking for other mechanisms of cell apoptosis induced by γ radiation.
出处
《中国肿瘤临床与康复》
2002年第6期1-3,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
