摘要
Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.
Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2002年第6期780-782,共3页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家重点基础研究发展规划 (973 )项目 (G 19990 5 5 90 0 2 )资助~~
