摘要
用白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）依赖细胞株Ｂ９的ＭＴＴ法，研究了４种刺激剂ＰＭＡ，Ａ２３１８７，ｆＭＬＰ和ＬＰＳ对小鼠腹腔巨噬细胞生成及分泌ＩＬ６的影响。分别检测了巨噬细胞培养上清液及胞溶部分ＩＬ６的含量。结果表明，ＰＭＡ（０１～１０μｍｏｌ·Ｌ－１），Ａ２３１８７（００１～１μｍｏｌ·Ｌ－１），ｆＭＬＰ（００２５～２５μｍｏｌ·Ｌ－１）均能促进巨噬细胞生成及分泌ＩＬ６，且有量效关系。ＬＰＳ（０１～１０μｇ·ｍｌ－１）能增加巨噬细胞培养上清液中ＩＬ６的含量，但对胞溶部分无影响。结果提示：巨噬细胞ＩＬ６的生成及分泌与蛋白激酶Ｃ（ＰＫＣ）和钙离子通道活化有关；
In this paper, a bioassay method involving the interleukin 6 dependent murine hybridoma B9 cell line was used to detect the level of released and cellassociated interleukin 6 in resident peritoneal macrophages from the mouse. The phorbol ester, phorbol 12myristate 13acetate(PMA), the calcium ionophores A23187, the chemotactic peptide fMLP and lipopolysaccharide(LPS) were introduced in these experiments as stimulators. PMA, A23187 and fMLP were shown to induce a dosedependent increase of both released and cellassociated interleukin 6, whereas, LPS caused increase of interleukin 6 activity in supernatants of cultured peritoneal macrophages of the mouse. The effects of PMA and A23187 suggested that the pathway of PKC and calcium is involved in the production and secretion of interleukin 6. The effects of fMLP and LPS provided evidence that they participated in the process of inflammation. The difference between LPS and the other three stimulators in cellassociated interleukin 6 activity might suggest a different mechanism of actions of generation of interleukin 6.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第12期893-897,共5页
Acta Pharmaceutica Sinica
