摘要
本文基于PC12细胞模型研究大豆蛋白水解物(Soybean protein isolate hydrolysates,SPIHs)对神经元氧化损伤的保护作用。以大豆蛋白为原料,经过酶解和膜分离得到四种分子量不同的水解物,我们首先检测了SPIHs的抗氧化能力;然后用H2O2刺激P12细胞,建立神经元氧化损伤模型,并以适当浓度的SPIHs处理细胞,通过检测各种生物学指标评价对细胞氧化损伤的保护作用。结果显示,低分子量的SPIHs表现出最强的抗氧化活性;能够提高损伤细胞的存活率和抗氧化酶活力,减少乳酸脱氢酶(LDH)的释放量和丙二醛(MDA)的生成,抑制细胞活性氧(ROS)的累积(p<0.05或p<0.01),且变化呈现一定的剂量依赖关系。研究认为,低分子量的SPIHs对神经元氧化损伤具有保护作用,可以作为功能性成分用于保护神经元氧化损伤相关的功能食品和保健品的开发。
The neuro-protective effects of soybean protein isolate hydrolysates(SPIHs) against neuronal oxidative damage were investigated in a PC12 cell model in this study. Four hydrolysates with different molecular weights were obtained from soybeans(raw material) through enzymatic hydrolysis and membrane separation. The antioxidant properties of the SPIHs were also investigated. Subsequently, a neuronal oxidative damage model was constructed by stimulating PC12 cells with H2O2. SPIHs at appropriate concentrations were used to treat the damaged cells; the effect of SPIHs on cellular oxidative damage was evaluated using various biological indices. The results of these analyses indicated that low molecular weight SPIHs exhibited the most potent antioxidant activities, and caused a dose-dependent improvement in the neuronal cell viability, reduction in lactate dehydrogenase(LDH) release and malondialdehyde(MDA) formation, and suppression of intracellular accumulation of reactive oxygen species(ROS)(p < 0.05 or p < 0.01). Based on the results of this study, low molecular weight SPIHs were believed to protect neuronal cells against neuronal oxidative damage, and could be utilized as a functional component in functional food and health products to protect against neuronal oxidative damage.
出处
《现代食品科技》
EI
CAS
北大核心
2015年第4期8-12 57,共6页
Modern Food Science and Technology
基金
国家科技支撑计划项目(2012BAD33B03)
吉林大学研究生创新基金资助项目(2014116)
关键词
大豆蛋白水解物
PC12细胞
氧化应激
细胞毒性
soybean protein isolate hydrolysates(SPIHs)
PC12 cells
oxidative stress
cytotoxicity
