摘要
目的 探讨铝盐的发育毒性及机理。方法 妊娠 9 5d大鼠胚胎于体外培养系统中给予不同剂量的硫酸铝 ,培养 4 8h后 ,观察胚胎生长发育和器官形态分化状态。结果 随着铝剂量的增加 ,反映胚胎生长发育和器官分化的各项指标呈现出下降趋势 ,有一定的剂量效应关系。其中 ,卵黄囊及血管网发育分化、心脏发育及体翻转等指标对铝的作用较敏感 ,铝剂量为 1 2 μg/ml时与对照比较已有显著性差异 (P <0 0 5 )。大于或等于 3 0 μg/ml时对各项指标的影响更为显著 (P <0 0 5或P <0 0 1) ,同时胚胎畸形发生率明显升高 (P <0 0 5 ) ,主要表面为神经管闭合不全、脑发育不良和体翻转不全。结论 Al2 (SO4) 3 有一定的胚胎毒性和致畸性。
ObjectiveTo discuss developmental toxicity and mechanism of aluminum.MethodsEmbryos of SD rat on day 9.5 of gestation were explanted and cultured in a whole-embryo culture system with exposure to Al 2(SO 4) 3 at Al 3+ concentrations for 48 hrs in order to understand the developmental toxicity of aluminum.After 48hrs in culture,each viable embryo was evaluated by Brown's morphological scoring system.Yolk sac diameter,crown-rump length,head length and embryonic dry weight were measured.ResultsDose-dependent decreases in embryonic development parameters and Brown's scores were observed with Al 3+ concentration.Yolk sac diameter,dry weight,neural tube,heart and flexion scores decreased significantly at 1.2μg/ml( P <0.05).When exposure doses at ≥3.0μg/ml,the embryonic development and morphogenetic differentiation were obviously inhibited( P <0.05 or P <0.01),as well as the incidence of dysmorphogenesis significantly increased,including dorsiflexion and neural tube defects.ConclusionAluminum was a developmental toxicant and dysmorphogent in rat.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第12期1431-1432,共2页
Chinese Journal of Public Health
