摘要
根际细菌丰富多样,对植物的生长发育有重要影响。为更好的了解野生蒙桑和移植栽培蒙桑根际细菌的多样性组成和差异,本研究提取了两样本的宏基因组DNA,利用Roche 454 GS FLX测序技术对样本菌群的16S r RNA基因的V1~V3区域进行测序。测序结果表明:野生蒙桑根际细菌的主导类群为变形菌门(31.62%)和酸酐菌门(19.8%);栽培蒙桑的主导类群为厚壁菌门(89.07%),两样本的沙浓指数分别为5.8和1.33。栽培蒙桑样本OTU498的基因序列数占总样本的78.9%,其最相近菌属为苏云金芽孢杆菌;野生蒙桑OTU656、OTU556、OTU568和OTU665占总样本的8.17%,最相近菌属是丝状共生菌。进化分析发现两样本菌群具有各自的特异性,大都来源于同一菌门的不同菌属,分别聚类。本研究表明移栽后蒙桑根际细菌的多样性降低,主导类群也发生了变化,基于16S r RNA测序可以揭示根际细菌的组成结构。
Plant rhizosphere is rich in variety of bacteria,which have an important influence on the growth and development of plants. In order to better understand the diversity and difference of rhizosphere bacterias between the wild and cultivated Morus mongolica,we extracted the metagenomic DNA and analyzed the rhizosphere microflora of two samples using the Roche 454 sequencing technology. The rhizosphere bacteria were characterized by amplifying the V1~V3 region of bacterial 16 S r RNA gene. The results showed that the dominant taxa in wild Morus mongolica Rhizosphere bacteria are Proteobacteria(31.62%) and Acidobacteria(19.8%),while in the cultivated sample,the dominant taxa were Firmicutes(89.07%). The Shannon index were 5.8 and 1.33 in the wild Morus mongolica rhizosphere bacteria and cultivated one. In cultivated Morus mongolica sample,78.9% of the total sequence belongs to OTU498,the closest genus of which is Bacillus thuringiensis. The four group of OTU656,OTU556,OTU568 and OTU665 in wild Morus mongolica sample,which accounted for 8.17% of the total sequence,is similar to the Methylobacterium sp. the rhizosphere bacterias of the two sample belong to the same Phylum,but different genus. The results of this study demonstrated that the diversity of cultivated Morus mongolica rhizosphere bacteria is lowered than that in the wild sample,and the dominant taxa changed in the cultivated Morus mongolica rhizosphere bacteria,as well. The sequencing analysis based on the metagenomic DNA and 16 S r RNA gene can reveal the composition of rhizosphere bacteria.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第10期2161-2168,共8页
Genomics and Applied Biology
基金
陕西省青年科技新星项目(No.2013KJXX-96)
陕西省教育厅重点实验室科研计划项目(No.14JS003)共同资助
关键词
蒙桑
根际细菌
16S
RRNA
多样性
Morus mongolica,Rhizosphere bacteria,16S r RNA,Diversity
