摘要
本研究以木薯品种新选048诱导膨大的茎尖分生组织为外植体,研究了不同浓度植物生长调节剂、不同碳源、不同成熟时间的体细胞胚及其子叶切片对木薯体细胞胚发生及植株再生的影响。结果表明,与2,4-D相比,12 mg/L的Picloram诱导木薯体细胞胚发生率最高,为36.2%,其产胚量为5.87;不同碳源对木薯体细胞胚继代增殖无显著性差异;随着体细胞胚成熟时间的增加,其植株的再生率也随之升高,但后期差异不显著;不同成熟时间的体细胞胚子叶器官发生率差异显著,诱导成熟14 d左右的子叶切片植株再生率较高,诱导率达54.3%。
This research taking swollen stem apical meristem of cassava(cv. Xin Xuan048) as explants, studied the effects of different plant growth regulators, carbon sources, mature embryo and cotyledons slice on the induction of somatic embryogenesis and plant regeneration. The results showed that the effects of Picloram were better than that of 2,4-D. The effects of 12 mg/L Picloram were highest, the incidence of somatic embryos was 36.2% and Embryo production quantity was 5.87; The effects of different carbon sources were non-significant; With the ripening time of somatic embryo increased, the incidence of plant regeneration increased. After 14 d embryo maturation, the incidence was non-significant, the ripening time of somatic embryo had a great influence on the organogenesis of cotyledon, frequency of cotyledon organogenesis was highest around 14 d after embryo maturation and the induction could be 54.3%.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第7期1522-1526,共5页
Genomics and Applied Biology
基金
国家自然科学基金项目"基于TILLING技术的变性淀粉木薯种质创新研究"(31301378)
广西壮族自治区"亚热带作物分子育种"八桂学者专项经费(桂人才通字(2011)4号)
广西农业科学院学科团队建设重点项目"木薯综合育种技术研究与新品种选育"(桂农科2015YT61)共同资助
关键词
木薯
体细胞胚发生
植株再生
Cassava,Somatic embryogenesis,Plant regeneration
