摘要
水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,以下简称Xoo)是水稻病原菌的一种,其引起的水稻白叶枯病(bacterial leaf blight,BLB)可致使水稻大量减产。本研究发现一个假定的udg H基因的插入突变致使致病力急剧下降,并且胞外多糖、抗逆性、生物膜、游动性等几种生理表型与野生型相比发生了明显变化。互补菌株的致病力能恢复至80%以上,其他生理表型基本能恢复至野生型。本研究为进一步研究udg H基因的致病分子机理提供了研究线索。
Bacterial leaf blight(BLB) caused by gram-negative bacterium Xanthomonas oryzae pv. oryzae(Xoo) is a devastating rice disease in the world. In our preliminary study, a random Tn5 transposon-mediated mutation library of Guangxi Xoo strain K74 was generated. Here, we characterized an udg H mutant of Xoo K74 and showed that the udg H mutant resulted in a reduction in virulence on the host. We also demonstrated that udg H is involved in cell motility, biofilm formation ability and polysaccharide synthesis. Furthermore, the udg H mutant showed increased sensitivity to hydrogen peroxide and SDS. All these phenotype in complementary strain can be restored to wild-type levels but virulence only around 80%. This work laid a solid foundation for the further study on pathogenic molecular mechanism of udg H.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第6期1218-1224,共7页
Genomics and Applied Biology
基金
农业部行业专项(201303015)
微生物及植物遗传工程教育部重点实验室开放课题(J1301)
广西大学科研基金资助项目(XGZ130088)
广西科技攻关项目(桂科合13999001
桂科能1347006-1)共同资助
关键词
水稻白叶枯病菌
UDP-脱氢酶
致病力
Xanthomonas oryzae pv.oryzae,UDP-glucose dehydrogenase,Virulence
