摘要
用60Co酌射线辐照粳稻"嘉花1号",并从其后代中筛选到一个新的苗期白化致死突变体asl4(albino seedling lethality 4),该突变体从发芽后至3叶期表现白化,3叶期后逐渐死亡。与野生型"嘉花1号"相比,asl4突变体的叶绿素与类胡萝素含量几乎为零。遗传分析表明,该突变性状受一对隐性核基因(asl4)控制。利用asl4突变体与广占63S杂交获得的F2、F3分离群体进行基因定位,应用微卫星(SSR)以及In Del分子标记将asl4基因定位在水稻第9染色体上的ID12974和ID13162分子标记之间的188 kb内,该区域内尚未发现与水稻叶绿素合成或叶绿体发育相关的已知功能基因。因此,推测ASL4基因是一个苗期生长发育关键基因。
A novel albino lethal mutant, asl4(albino seedling lethality 4), was selected from the progeny of a japonica rice variety 'Jiahua 1'treated with 60Co酌-radiation. The asl4 mutant showed the albino phenotype from germination to 3-leaf-stage, then died. In comparison with the wild-type parent, 'Jiahua 1', the chlorophyll and carotenoid contents in asl4 mutant was hardly detectable. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene(asl4). In addition, with the help of SSR, new developed In Del markers and F2 and F3segregating population derived from the cross between the asl4 mutant and the indica rice Guangzhan 63 S, the asl4 was narrowed to 188 kb between the mo1 ecular markers ID12974 and ID13739 on chromosome 9, in which known functional genes for chlorophyll synthesis or chloroplast development in rice have not been found. Thus, the rice ASL4 might be a key gene for growth and development at the seedling stage.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2014年第6期1190-1195,共6页
Genomics and Applied Biology
基金
国家自然科学基金(30971552)
上海市科委项目(10DZ2271800
12ZR1422000
09DJ1400505
0939191200)
上海市教委项目(J50401)共同资助
关键词
水稻
白化
遗传分析
基因定位
Rice(Oryza sativa),Albino,Genetic analysis,Gene mapping
