摘要
本文叙述了测定人白细胞介素2(IL-2)含量的ELISA双抗体夹心法。用辣根过氧化物酶标记IL-2单克隆抗体。聚苯乙烯反应板预先用0.1％戊二醛处理,然后以单克隆抗体包被。本法批内变异系数CV=3.99％,批间变异系数CV=5.96％,标准曲线的测定范围为3.1～100u/ml,检测下限为1.55u/ml。应用本法已测定了2S例正常人和11例恶性肿瘤患者的IL-2含量。
In the present experiment, a double antibody sandwich ELISA method for measurement of human interleukin-2 (IL-2 ) was described. The monoclonal antibody against IL-2 was labelled with horseradish peroxidase. The polystyrene plates were pretreated with 0.1% glutaraldehyde and coated with the monoclonal antibody. Within run and between run variation coefficients of the assay were 3.9% and 5.96%, respectively. The working range of the standard curve for IL-2 was 3. l-100u/ml and the minimal detectable limit was 1.55u/ml. The level of IL-2 in 25 normal subjects and 11 cancer patients were determined using the assay.
