摘要
经磷缓液提取 ,乙醇沉淀 ,超滤去杂 ,然后经CM -纤维素柱层析 ,DEAE -SephadexA -5 0柱层析纯化了Dactylosporangiumaurantiacum菌脲酶 ,纯化倍数达 5 0 .0 1 ,活力回收率为 1 7.81 %。经SDS -PAGE垂直平板电泳测得其分子量为 42 0 0 0。经氨基酸组成分析 ,该酶含 2 71个氨基酸残基 ,该酶的紫外吸收峰为2 75nm。
The urease extracted from Dactylosporangium aurantiacum with phosphate-buffered saline, precipitated by ethancl, decontaminated through ultifiltration, purified by cm-cellulose chromatography and DEAE-sephadex A50 column chromatography, the purification times reached 50.01 and the recovery ratio of activity was 17.81%. The enzyme's molecular weight was 42000 measured by SDS-PAGE vertical slab gel electrophoresis. The amino acid composition analysis showed that this enzyme is composed of 271 aminc acid residues, and that its ultraviolet absorption peak is 275nm.
出处
《江西农业大学学报》
CAS
CSCD
2002年第5期595-602,共8页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金资助项目 (3 9760 0 3 7)
江西省自然科学基金资助项目 (0 0 3 0 0 3 8)
