摘要
目的检测原发性胃腺癌(gastric adenocarcinoma,GAC)中上皮间充质转化(epithelial mesenchymal transition,EMT)相关标记物Twist和Snail蛋白以及肿瘤转移抑制因子KAI1水平,并分析它们之间的相互关系以及临床意义。方法通过免疫组织化学Elivision^(TM)plus法检测261例GAC和80例正常胃黏膜组织中Twist、Snail和KAI1蛋白的免疫反应性。结果Twist和Snail在GAC组织中的免疫反应阳性率显著高于其在对照组中的阳性率;KAI1蛋白在GAC组织中的免疫反应阳性率显著低于其在对照组中免疫反应阳性率。Twist和Snail蛋白免疫反应阳性率与GAC组织的浸润深度、临床病理分期和淋巴结转移均显著相关;KAI1蛋白的免疫反应阳性率与GAC肿瘤组织的分化程度、浸润深度、临床病理分期及淋巴结转移显著相关。Kaplan-Meier生存分析显示,Twist和Snail蛋白免疫反应阳性组患者的总体生存时间明显短于其阴性组患者,而KAI1蛋白免疫反应阳性组的患者总体生存时间明显长于其阴性组患者。Spearman相关分析显示,KAI1蛋白与Twist和Snail蛋白的免疫反应性之间呈负相关,Twist和Snail的免疫反应性呈正相关。Cox多因素回归分析表明Twist和KAI1蛋白的免疫反应阳性以及pTNM分期是影响GAC患者生存的独立预后因素。结论 Twist、Snail水平升高和KAI1水平降低可能参与了GAC的浸润及转移过程;联合检测Twist、Snail和KAI1的免疫反应性对GAC患者的肿瘤进展及预后判断有重要意义。
Objective To detect the immunoreactivity of the epithelial mesenchymal transition(EMT) related markers of Twist and Snail and that of the tumor metastasis suppressor KAIl in primary gastric adenocarcinoma(GAC),and explore their association with the clinicopathological characteristics.Methods Immunoreactivity of Twist,Snail,and KAIl protein was investigated by immunohistochemistry in 261 specimens of GAC tissues and 80 specimens of normal gastric tissues(as the control group).Results The positive rate of immunoreactivity of Twist and Snail was significantly higher in GAC tissues than in control tissues;and that of KAIl was significantly lower in GAC than in control tissues.The positive rate of immunoreactivity of Twist and Snail was significantly related to invasion,lymph node metastasis,and pTNM stage.There was a negative association between the immunoreactivity of KAIl protein and differentiation grade,invasion,lymph node metastasis,and pTNM stage.Kaplan-Meier survival analysis showed that the survival time of patients with positive Twist and Snail was significandy shorter than that of negative patients;and the survival time of patients with positive KAIl was significandy longer than that of negative patients.Spearman analysis showed that there was a negative correlation between the positive immunoreactivity of KAIl and that of Twist or Snail;and there was a positive correlation between the positive immunoreactivity of Twist and that of Snail.COX multivariate analysis showed that die positive immunoreactivity of Twist and KAIl protein,and pTNM stage were independent prognostic factors of GAC patients.Conclusion The abnormal immunoreactivity of Twist,Snail,and KAIl may be involved in the development,invasion,and metastasis of GAC.The combined detection of Twist,Snail,and KAIl has an important role in predicting the progression and prognosis of GAC.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2016年第2期136-142,共7页
Chinese Journal of Histochemistry and Cytochemistry
基金
安徽省高等学校自然科学研究项目(KJ2015B099by)
蚌埠医学院自然科学研究项目(byky1337)
