摘要
目的 研究双色双融合探针间期荧光原位杂交技术 (D FISH)检测慢性髓系白血病(CML)微小残留病 (MRD)的灵敏度、特异性及可重复性。方法 应用D FISH探针对 8例移植后或经供者淋巴细胞输注治疗获完全细胞遗传学缓解的CML患者骨髓内残留的微小肿瘤负荷水平进行检测 ,与应用单融合探针 (S FISH)进行了比较 ,同时就D FISH与逆转录 聚合酶链反应 (RT PCR)结果的相关性进行了探讨。结果 D FISH较S FISH具有更高的灵敏度及特异性 ,可重复性好。正常对照组中D FISH的正常分界值为 0 5 70 % ,而S FISH为 5 686% ,差异具有显著性 ;对 8例移植后患者进行MRD检测S FISH检测到 3 / 8(3 7 5 % )阳性 ,而D FISH检测到 5 / 8例 (62 5 % )阳性且结果与RT PCR结果高度相关。结论 由于D FISH具有较低的假阳性率与假阴性率 ,可应用于CML治疗后MRD的检测 。
Objective To explore the sensitivity, specificity and reproducibility of dual color and dual fusion fluorescence in situ hybridization (D FISH) in detecting the minimal residual disease (MRD) of chronic myeloid leukemia (CML) after treatment Methods MRD were detected in the bone marrow of 8 cases of chronic myeloid leukemia after treatment with interphase fluorescence in situ hybridization Sensitivity, specificity and reproducibility of D FISH were compared with those of single fusion FISH (S FISH) Furthermore, correlation between the results of D FISH and RT PCR was explored Results D FISH was more sensitive and specific than S FISH and had a good reproducibility In normal control, the cutoff rates of D FISH and S FISH were 0 570% and 5 868% with significant statistic difference In detection of MRD in 8 cases of CML post transplantation, positive rates of S FISH and D FISH were 3/8(37 5%) and 5/8(62 5%) respectively Furthermore, the results of D FISH had a high correlation with that of RT PCR Conclusion In view of the lower false positive and false negative results of D FISH, it may be used in the detection of minimal residual disease in CML after treatment But further exploration is required for verifying the correlation between the results of D FISH and the clinical parameters
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第12期801-804,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助项目 (39770 831)
广东省自然科学基金资助项目 (970 833)
