摘要
从栽培海区采回的发病条斑紫菜 (Porphyraeyezoensis)上共分离筛选出 5 1株菌株 ,全部进行人工感染 ,其中 17# 菌株发病症状最典型 ,而感染对照组没有可见变化。初步确定 ,17# 菌株是条斑紫菜绿斑病的主要致病菌。17# 菌株为短杆状、极生单鞭毛可运动的革兰氏阴性菌 ,产生紫褐色色素 ,可水解明胶、酪蛋白、淀粉等大分子物质。葡萄糖氧化发酵试验 (O/F)为氧化性、氧化酶阳性、ONPG反应阳性 ,V -P反应阴性 ,生长需Na+ 等生化特性 ,G +C含量 4 0 .5mol% ,脂肪酸主要成分为C17∶1ω8c和C16∶1ω7c。基于 16SrRNA的系统进化分析均表明 ,从患绿斑病的条斑紫菜叶状体上分离到的致病菌株 17# 与柠檬假交替单胞菌 (Pseudoalteromonascitrea)
The Porphyrae yezoensis samples with green spot disease were collected from the southwest coast of Jiangsu Province. From the diseased lavers, 51 strains were separated and infected to the health ones artificially, among which a strain named Strain 17 had the most typical disease symptom like the natural diseased lavers. Strain 17 is aerobic and gram negative with polar flagella, which can produce some dark brown pigment and hydrolyze gelatin, casein and starch. The strain is also positive for oxidase ONPG and negative for V P, and Na + is essential for it to grow. The content of G+C in this strain is 40.5 mol % and C17∶1ω8c and C16∶1ω7c dominant the total lipid acids. The tree in terms of 16S rRNA systematic analysis indicates that Strain 17 has a close relationship with Pseudoalteromonas citrea that they have a sequence similarity of 99.4% . It is concluded that the Strain 17 belongs to P. citrea but different strain.
出处
《中国水产科学》
CAS
CSCD
北大核心
2002年第4期353-358,共6页
Journal of Fishery Sciences of China
基金
农业部
财政部首批农业科技跨越计划项目 (1999-2 0 )
关键词
条斑紫菜
绿斑病
柠檬假交替单胞菌
Porphyrae yezoensis
green spot disease
Pseudoalteromonas citrea
