摘要
为研究尾悬吊模拟失重大鼠肺组织细胞凋亡调控基因的变化 ,采用尾悬吊模拟失重 ,分为悬吊 7天和 2 1天及相应对照 4组 ,每组 10只健康雄性SD大鼠 ,并采用原位杂交技术检测肺组织bcl 2 /bax的表达情况。发现 7天尾悬吊模拟失重大鼠肺组织bcl 2mRNA水平明显低于对照组 ,两者之间差异有显著性意义 (P <0 0 5 ) ,但 2 1天bcl 2mRNA水平与对照组相比无显著性差异 (P >0 0 5 ) ;7天尾悬吊模拟失重大鼠肺组织baxmRNA水平明显高于对照组 ,两者之间差异有显著性意义(P <0 0 5 ) ,但 2 1天baxmRNA水平与对照组相比亦无显著性差异 (P >0 0 5 )。提示尾悬吊 7天模拟失重大鼠肺组织细胞凋亡调控基因存在变化。
To investigate the changes of cell apoptosis genes in pulmonary tissue of tail suspended rats during simulated weightlessness, 40 Spragno Dawley male rats were divided into 4 groups: suspended for 7d,control of 7d, suspended for 21d, and control of 21d.Tail suspension(-30°) was used as weightlessness simulation.The condition of bcl 2/bax in pulmonary tissues was detected by in situ hybridization. The results showed that the level of bcl 2 mRNA in pulmonary tissue of 7d tail suspended rats decreased significantly( P <0 05) and that of 21d ones did not change prominently( P >0 05). The results also showed that the level of bax mRNA in pulmonary tissue of 7d tail suspended rats increased significantly( P <0 01) and that of 21d ones did not change prominently( P >0 05).It can be concluded that there are certain changes of cell apoptosis genes in pulmonary tissue of 7d tail suspended rats during simulated weightlessness.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第11期1012-1013,共2页
Medical Journal of Chinese People's Liberation Army
基金
武器装备预研基金项目资助课题 (编号 51 4 2 2 0 30 1 0 1JB1 0 0 2 )
