摘要
丝瓜为葫芦科丝瓜属作物,含有丰富的营养物质,深受消费者喜欢,是我国主要的瓜类蔬菜之一。随着丝瓜种质资源的数量和类型不断增加,丝瓜优异材料的筛选、保存难度日益增大。为了构建丝瓜核心种质库,剔除同质化严重的种质材料,提高丝瓜育种效率,本研究以丝瓜极具代表性的18个表型性状为指标,对244份丝瓜种质资源的遗传多样性进行研究,以变异系数变化率、香农指数、表型保留比例等7项参数为评价指标,对比分析8种不同抽样比例(5%~40%)、3种不同取样方法(随机聚类取样、分组聚类取样、优先聚类取样)的丝瓜候选核心种质的代表性。结果发现:丝瓜种质资源的表型遗传变异较为丰富,其中香农指数最大的是叶宽,为3.0,最小的是种瓜长,为1.8。利用7个评价指标综合分析发现最适宜的抽样比例为20%,最适宜的取样方式为分组聚类取样。利用该方法进行核心种质库构建,获得的初级核心种质包含51份丝瓜种质资源,占全部原始种质资源的20.90%,与原始种质对比,18个农艺性状中有13个性状与原种质的极大值、极小值完全一致,大部分性状的特征值与原种质相同或是差异较小,经T检验发现所有表型性状均值与原种质无显著差异,表明采用该方法构建的核心种质库具有较好的代表性,遗传多样性丰富。本研究可为后期丝瓜种质资源的收集、保存和创新利用提供基础支撑,同时也为提高丝瓜品种改良效率奠定基础。
Luffa,a major melon vegetables in China,is a crop of gourd family and the silk gourd genus,which is rich in nutrients and favored by consumers.With the increasing number and type of luffa germplasm resources,it is increas-ingly difficult to screen and preserve the excellent materials of luffa.In order to construct a core germplasm bank of luffa,eliminate germplasm materials with serious homogenization,and improve the efficiency of lucerne breeding,this study investigated the genetic diversity of 244 luffa germplasm resources by using 18 phenotypic traits that are highly representative of luffa as indicators,and compared and analyzed eight different sampling ratios(5%-40%),and three different sampling methods(random cluster sampling,group cluster sampling and preferential cluster sampling)were compared to analyze the representativeness of the core germplasm of luffa candidates.The results showed that the phe-notypic genetic variation of lucerne germplasm resources was relatively rich,of which the largest Shannon index was leaf width,3.0,and the smallest was seed melon length,1.8.The most suitable sampling ratio was 20%using the com-prehensive analysis of the seven evaluation indexes,and the most suitable sampling method was group cluster sampling.Using this method for core germplasm library construction,the primary core germplasm obtained containing 51 lucerne germplasm resources,accounting for 20.90%of all the original germplasm resources,and compared with the original germplasm,13 of the 18 agronomic traits were in full agreement with the original germplasm in terms of the maximum and minimum values,and the characteristic values of most traits were the same as those of the original germplasm or the differences were relatively small.The T-test showed that the mean values of all phenotypic traits had no significant dif-ferences with the original germplasm,indicating that the core germplasm library constructed by the method has good representativeness and rich genetic diversity.This study could provide basic suppor
作者
张婷
王雪艳
郭勤卫
韦静
方萍萍
谢利芬
赵东风
李朝森
ZHANG Ting;WANG Xueyan;GUO Qinwei;WEI Jing;FANG Pingping;XIE Lifen;ZHAO Dongfeng;LI Chaosen(Quzhou Academy of Agricultural and Forestry Sciences,Quzhou,Zhejiang 324000,China)
出处
《热带作物学报》
北大核心
2025年第1期106-114,共9页
Chinese Journal of Tropical Crops
基金
浙江省自然科学基金项目(No.LGN21C150001)
衢州市科技攻关项目(No.2023K083)。
关键词
丝瓜
表型性状
核心种质
Luffa spp.
phenotypic characters
core germplasm
