摘要
目的响应面法优化高良姜二苯基庚烷类成分的提取工艺,并评价其体外抗氧化活性。方法采用二氯甲烷冷浸法提取高良姜二苯基庚烷类成分。在单因素试验基础上,以提取次数、提取时间、料液比为影响因素,以二苯基庚烷类成分提取率为指标,应用Box-Behnken响应面法分析各因素对提取率的影响,得出高良姜二苯基庚烷类成分的最佳提取条件。以Fe3+还原能力和总抗氧化能力(ABTS法)评价高良姜二苯基庚烷类成分体外抗氧活性。结果最佳提取工艺为:提取时间56 h,料液比1∶12,提取次数2次,二苯基庚烷类成分提取率为2.883%,RSD为1.05%。高良姜二苯基庚烷类成分提取物浓度为0.25 mg/mL时,Fe3+还原能力和总抗氧化能力(ABTS法)都有最大值,分别为2.49 mmol·g^(-1)和2.10 mmol·g^(-1)。结论优化的高良姜二苯基庚烷类成分提取工艺稳定、可行、简便,所得提取物在体外表现出较好的抗氧化能力。
Objective To optimize of extraction process of diphenylheptane from Gaoliangjiang(AlpiniaOfficinarumRhizoma)by response surface methodology and study its antioxidant activity in vitro experiments.Methods The diphenylheptane of Gaoliangjiang(AlpiniaOfficinarumRhizoma)was extracted by cold soaking with dichloromethane.With extraction times,extraction time and solid-liquid ratio as the variables,The extraction rate of diphenylheptane of Gaoliangjiang(Alpinia Officinarum Rhizoma)as the index,Box-Behnken response surface methodology was used to analyze the influence of various factors on extraction rate and determine the optimal extraction conditions.The antioxidant capacity of diphenylheptane from Gaoliangjiang(Alpinia Officinarum Rhizoma)was evaluated by Fe3+reduction capacity and the total antioxidant capacity(ABTS method)Results The optimal extraction process:extraction time of 56 hours,solid-liquid ratio of 1∶2,extraction frequency of 2 times.The extraction rate of diphenylheptane was 2.883%with RSD of 1.05%.When the concentration of diphenylheptane extract from Gaoliangjiang(Alpinia OfficinarumRhizoma)was 0.25 mg/mL,the maximum value of Fe3+reduction ability and total antioxidant capacity(ABTS method)were 2.49 mmol·g^(-1) and 2.10 mmol·g^(-1),respectively.Conclusion The optimized extraction process of diphenylheptane from Gaoliangjiang(Alpinia Officinarum Rhizoma)is stable and feasible and simple.The diphenylheptane extract of Gaoliangjiang(Alpinia Officinarum Rhizoma)has good antioxidant capacity in vitro.
作者
蒙宏杰
陈美安
黄燕琼
陈卓尔
江芳静
覃金桥
陈智兰
赵天琪
秦华珍
MENG Hongjie;CHEN Meian;HUANG Yanqiong;CHEN Zhuoer;JIANG Fangjing;QIN Jinqiao;CHEN Zhian;ZHAO Tianqi;QIN Huazhen(Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China)
出处
《辽宁中医药大学学报》
CAS
2025年第1期51-55,F0003,共6页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金(82074034)。
关键词
高良姜
二苯基庚烷
响应面
抗氧化
Gaoliangjiang(Alpinia Officinarum Rhizoma)
diphenylheptane
response surface methodology
antioxidation
