摘要
伪结核棒状杆菌(Cp)是羊体表脓肿及干酪样淋巴结炎(CLA)的重要病原,接种疫苗是防控Cp感染致病最有效的手段之一。以Cp万州株(WZ)作为疫苗株,甲醛为灭活剂,ISA 201 VG为佐剂制备灭活苗,对山羊进行免疫,首免后28 d进行二免,二免后30 d以WZ株攻毒,连续观察65 d。通过检测免疫和攻毒山羊的增质量、体温、血常规、血清免疫球蛋白G(IgG)水平、淋巴结脓肿数量及载菌量等,综合评价Cp灭活苗的免疫保护效果。结果显示:与对照组相比,受免山羊血清IgG抗体在首免后第3、4周和二免后第2~4周均显著升高,且二免IgG抗体明显高于首免。攻毒发现,对照组山羊体温在攻毒后7 d内均高于免疫组,攻毒后1~8周内对照组山羊增质量均低于免疫组,且免疫组山羊脓肿数量及Cp数均明显少于对照组,提示该灭活苗免疫可缓解因Cp感染引起的山羊体质量下降及体温升高,减少Cp攻毒引起的脓肿数量和淋巴结Cp载菌量。综合表明:所制备Cp灭活苗可提高山羊的免疫水平,对该病原感染具有较好的保护作用。
Corynebacterium pseudotuberculosis(Cp)is an important pathogen responsible for surface abscesses and caseous lymphadenitis(CLA)of goats,and vaccination is one of the most effective methods to prevent and control Cp infection.In this study,Cp Wanzhou strain(WZ)was used as the vaccine candidate,formaldehyde as the inactivating agent,and ISA 201 VG as the adjuvant to prepare an inactivated vaccine.The goats were immunized with Cp vaccine for twice with 28 d interval,and were challenged with Cp WZ at 30 th days after the second immunization.The goats were continuously observed for 65 d.The immunoprotective effect were evaluate by detecting the weight gain,temperature,routine blood,serum IgG levels,the number of lymph node abscesses and the bacterial load in the goats.The results showed that compared with the control group,the serum IgG antibodies of the immunized goats significantly increased at the 3 rd and 4 th weeks after the first immunization and 2-4 weeks after the 2 nd immunization,and the IgG antibodies of the 2 nd immunization were significantly higher than those of the 1 st immunization.The temperature of goats in control was higher than that of the immuned goats within 7 days after the challenge.The weight gain of goats in control was lower than that of the immuned ones within 1-8 weeks,and the number of abscesses and Cp in the immuned goat group were significantly lower than that of the control.The above results suggested that inactivated vaccine immunization could alleviate the weight loss and the body temperature increase caused by Cp infection in goats,reduce the number of abscesses and Cp bacterial load in lymph node caused by Cp infection.In summary,the prepared Cp inactivated vaccine could improve the immune level of goats and had a good protective effect against Cp infection.
作者
邹安龙
吕红
吴婵玉
李鑫灿
宋艳
钱虹宇
王芝英
周作勇
ZOU Anlong;LYU Hong;WU Chanyu;LI Xincan;SONG Yan;QIAN Hongyu;WANG Zhiying;ZHOU Zuoyong(College of Veterinary Medicine,Southwest University,Rongchang Chongqing 402460,China)
出处
《西南大学学报（自然科学版）》
CAS
北大核心
2025年第1期104-111,共8页
Journal of Southwest University(Natural Science Edition)
基金
重庆市自然科学基金项目(cstc2021jcyj-msxmX0884)
重庆市技术创新与应用示范项目(cstc2018jscx-msybX0216)。
关键词
伪结核棒状杆菌
灭活苗
山羊
免疫效果
Corynebacterium pseudotuberculosis
inactivated vaccine
goats
immune effect
