摘要
目的:采用液相色谱-串联质谱(LC-MS/MS)建立人参药材中30种禁用农药残留含量测定的分析方法。方法:采用直接提取法,样品经乙腈提取,先后经匀浆,离心,最后浓缩以乙腈定容,以基质外标法进行结果定量。结果:30种禁用农药按照定量限分为5组,A组(定量限为0.01mg/kg)在2~25ng/ml、B组(定量限为0.02mg/kg)在4~50ng/ml、C组(定量限为0.03mg/kg)在6~75ng/ml、D组(定量限为0.05mg/kg)在10~125ng/ml、E组(定量限为0.1mg/kg)在20~250ng/ml的线性关系均良好,相关系数均可达到0.993以上,平均回收率在60%~110%,相对标准偏差在1.1%~11.2%。结论:使用该方法检测人参药材中的30种农药残留准确度高、重复性好、便捷高效。
Objective:This study aims to establish an analytical method for determining 30 prohibited pesticide residues in ginseng medicinal materials using liquid chromatography with tandem mass spectrometry.Method:A direct extraction method was applied.The samples were extracted with acetonitrile,followed by homogenization,centrifugation,and final concentration to a fixed volume with acetonitrile.The results were quantified using the matrix external standard method.Results:The 30 prohibited pesticides were categorized into 5 groups according to their quantitative limits.Group A(quantitative limit:0.01mg/kg)showed a linear range of 2-25ng/ml;Group B(0.02mg/kg)showed 4-50ng/ml;Group C(0.03mg/kg)showed 6-75ng/ml;Group D(0.05mg/kg)showed 10-125ng/ml;and Group E(0.1mg/kg)showed 20-250ng/ml.All correlation coefficients exceeded 0.993.The average recovery rate ranged from 60%to 110%,with a relative standard deviation(RSD)between 1.1%and 11.2%.Conclusion:This method demonstrates high accuracy,good repeatability,and efficiency for detecting 30 pesticide residues in ginseng medicinal materials.
作者
张志敏
罗志伟
ZHANG Zhi-min;LUO Zhi-wei(Nanping Food and Drug Inspection and Testing Center)
出处
《中国食品药品监管》
2024年第11期100-105,共6页
China Food & Drug Administration Magazine
关键词
人参
液相色谱-串联质谱:快速提取法
禁用农药
ginseng
liquid chromatography with tandem mass spectrometry
rapid extraction method
prohibited pesticide residues
