摘要
[Objectives] In order to develop Triplostegia glandulifera Wall.ex DC. into foromula granules, a high performance liquid chromatography (HPLC) determination method and a TLC identification method for loganic acid in decoction pieces, standard decoction and formula granules of T. glandulifera were established, and the quantity value transfer relationship of T. glandulifera pieces-standard decoction-formula granules was investigated.[Methods] An Agilent 1260 II high performance liquid chromatograph and a Waters Symmetry C 18 column (4.6 mm×250 mm, 5 μm) were used to perform gradient elution with acetonitrile-0.1%phosphoric acid solution as the mobile phase at a column temperature of 30 ℃ and a flow rate of 1 mL/min, and the detection wavelength was 240 nm. The TLC identification method of T. glandulifera was established using ethyl acetate-methanol-water (10 : 2 : 1) as the developer, and examination was carried out under a UV lamp (254 nm). The quantity value transfer law was analyzed by using the extract yield, the content of loganic acid and TLC chromatograms as the main evaluation indexes.[Results] The method for the determination and identification of loganic acid is stable, reproducible and reliable. The average yield, average loganic acid content and average loganic acid content transfer rate of 3 batches of T. glandulifera formula granules were, respectively, 18.7%, 41.1 mg/g and 43.1%, each of which was within corresponding range of mean±3SD of the 15 batches of standard decoction.[Conclusions] The content determination and TLC identification methods of loganic acid can be used to evaluate the quality of T. glandulifera formula granules. This study provides data basis for further research on T. glandulifera formula granules, and promotes the modernization of medicines for ethnic minorities.
基金
Supported by Yunnan Provincial Science and Technology Major Project(202102AA310027).
