摘要
目的制备五指毛桃饮片标准汤剂,并进行质量标准研究。方法采用水煎煮法将19批不同产地的五指毛桃饮片制成标准汤剂,考察标准汤剂的出膏率、补骨脂素和佛手柑内酯的含量及转移率,建立标准汤剂的高效液相色谱(HPLC)特征图谱,分别对19批五指毛桃饮片标准汤剂进行相似度评价。结果19批五指毛桃饮片标准汤剂的出膏率、补骨脂素含量、佛手柑内酯含量及这两个指标性成分的转移率分别为4.9%~12.4%、109.3~791.5μg/g、45.0~175.0μg/g、10.0%~35.0%和8.0%~35.0%。HPLC特征图谱共确定了8个共有峰,6号、7号峰分别为补骨脂素和佛手柑内酯峰。19批五指毛桃饮片标准汤剂的相似度大于0.95。结论本研究建立的五指毛桃饮片标准汤剂的质量标准准确、可靠,可为五指毛桃饮片标准汤剂、配方颗粒及相关制剂的质量控制提供参考。
Objective To prepare standard decoction of Fici Hirtae Radix medicinal slices and conduct quality standard research.Methods The standard decoction of 19 batches of Fici Hirtae Radix medicinal slices from different origins was obtained by water decocting method.The extraction rate of the standard decoction and the contents and transfer rate of psoralen and bergapten were evaluated,and the HPLC characteristic chromatogram was established.The similarity of 19 batches of Fici Hirtae Radix standard decoction was evaluated respectively.Results The extraction rates,psoralen content,bergapten content and the transfer rates of these two index components of 19 batches of standard decoction of Fici Hirtae Radix medicinal slices were 4.9%-12.4%,109.3-791.5μg/g,45.0-175.0μg/g,10.0%-35.0%and 8.0%-35.0%,respectively.A total of eight common peaks were identified by HPLC characteristic chromatogram,and peaks labelled 6 and 7 were peaks of psoralen and bergapten,respectively.The similarity of 19 batches of Fici Hirtae Radix standard decoction was greater than 0.95.Conclusion The quality standard of Fici Hirtae Radix standard decoction established in this study is accurate and reliable,which can provide reference for the quality control of the standard decoction,formula granules and related preparations.
作者
苏静红
梁永娟
李锦清
陈清
蒋林
林青华
SU Jing-hong;LIANG Yong-juan;LI Jin-qing;CHEN Qing;JIANG Lin;LIN Qing-hua(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《食品与药品》
CAS
2024年第6期547-553,共7页
Food and Drug
基金
广西重点研发计划项目(编号:桂科AB21196051)。
关键词
五指毛桃饮片
标准汤剂
质量标准
特征图谱
Fici Hirtae Radix medicinal slices
standard decoction
quality standard
characteristic chromatogram
