摘要
目的 建立基于UPLC-Q-TOF/MS技术的龙生蛭胶囊化学成分的快速检测与鉴别方法。方法 采用UPLC-Q/TOF-MS技术对龙生蛭胶囊的化学成分进行分析鉴定,色谱柱为Waters Acquity UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm),流动相A:0.1%甲酸水,流动相B为含有0.1%甲酸的甲醇乙腈(1∶1)溶液。流速为0.3mL/min,柱温为40℃,进样量2μL。离子源采用电喷雾离子源,正、负离子模式下采集数据,采集范围为m/z50~1200,通过UNIFI软件结合人工核对化学成分进行鉴定与匹配。结果 初步快速鉴定出了87种化学成分。结论 所建立的方法能够系统、快速地分析龙生蛭中的化学成分,为药效物质研究提供依据,对龙生蛭的质量控制具有重要意义。
Objective To establish a rapid detection and identification method for the chemical components of Longshengzhi capsules based on UPLC-Q-TOF/MS.Method UPLC-Q/TOF-MS technology was used to analyze and identify the chemical components of Longshengzhi capsules.The chromatographic column was a Waters Acquity UPLC BEH C18 column(100mm×2.1mm,1.7μm),and the mobile phase A was 0.1%formic acid water.The mobile phase B was a methanol acetonitrile(1∶1)solution containing 0.1%formic acid(B).The flow rate is 0.3mL/min,the column temperature is 40℃,and the injection volume is 2μL.The ion source adopts the electric spray ion source.The data is collected in the positive and negative ion modes,and the collection range is m/z 50~1200.The identification and matching are carried out through UNIFI software combined with manual verification of chemical components.Result 87 chemical components were preliminarily and rapidly identified.Conclusion The established method can systematically and rapidly analyze the chemical components in Longsheng leeches,providing a basis for the study of medicinal substances and having important significance for the quality control of Longsheng leeches.
作者
赵孟依
毕成浩
李梦娇
董政委
范家玮
张晋岳
ZHAO Mengyi;BI Chenghao;LI Mengjiao;DONG Zhengwei;FAN Jiawei;ZHANG Jinyue(The First Clinical College of Shanxi University of Traditional Chinese Medicine,Taiyuan 030000,China;School of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 301600,China;School of Pharmacy&Institute of Materia Medica,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Heart Center/National Regional(Traditional Chinese Medicine)Cardiovascular Diagnosis and Treatment Center,Zhengzhou 450000,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2024年第8期2144-2153,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
山西中医药大学附属医院国家区域中医医疗中心心血管专项基金(XGZG202119):黄龙通脉胶囊治疗出血性中风非热证型的临床观察,负责人:张晋岳。
关键词
龙生蛭胶囊
中药方剂
化学成分
高效液相色谱
四极杆飞行时间质谱
Longshengzhi capsules
Traditional Chinese medicine prescription
Chemical composition
High performance liquid chromatography
Quadrupole time-of-flight mass spectrometry
