摘要
近年来,养殖方斑东风螺(Babylonia areolata)体内的恩诺沙星残留问题频发,为揭示恩诺沙星及其代谢物在方斑东风螺体内的药代动力学规律,并制定出合理的给药方法与休药期。以方斑东风螺为研究对象,在(24.5±2.5)℃的养殖水温下,对方斑东风螺伴饵投喂20 mg·kg^(-1)的恩诺沙星混合液,分别于给药后的第0.17、第0.25、第0.5、第1、第2、第4、第12、第24、第48、第72、第120、第168、第240、第360小时分期采集组织,采用岛津8050LCMS/MS仪器检测各时期组织中的药物浓度。结果显示,药时数据适用于非房室模型,恩诺沙星在腹足肌肉、吻管(含食道腺)、肝脏中的达峰浓度(Cmax)分别为7.82、10.5、31.47 mg·kg^(-1),环丙沙星(代谢物)的Cmax分别为0.72、0.89、1.21 mg·kg^(-1);恩诺沙星的达峰时间(tmax)分别为1、4、4 h,环丙沙星的tmax分别为4、12、4 h;恩诺沙星的消除半衰期(t1/2z)分别为38.22、52.44、62.40 h,环丙沙星的t1/2z分别为66.23、33.11、27.06 h;各组织理论休药期分别为16.94、16.79、17.99 d。结果表明,各组织中Cmax/MIC>10,故20 mg·kg^(-1)的恩诺沙星混合液适用于治疗由哈维氏弧菌(Vibrio harveyi,MIC,0.45 mg·L^(-1))引发的东风螺疾病。研究建议休药期不低于440.76度日(℃·d),给药方案为每2天1次,共3次。
In recent years,frequent occurrence of enrofloxacin residues in breeding snail(Babylonia areolate)has been a problem.To reveal the pharmacokinetic characteristics of enrofloxacin and its metabolites in B.areolate,and to design a rational dosing method and the rest period,we fed B.areolate with 20 mg·kg^(-1) of enrofloxacin mixture at water temperature of(24.5±2.5)℃,and then sampled at 0.17th,0.25th,0.5th,1st,2nd,4th,12th,24th,48th,72nd,120th,168th,240th and 360th hour after the dosing.Shimadzu 8050 LC-MS/MS was used to determine the drug concentration in the tissues in each period.The results show that the drug-time data applied to the non-atrial models in Gastropod muscle,proboscis(Including esophageal glands)and liver:the Cmax of enrofloxacin was 7.82,10.5 and 31.47 mg·kg^(-1),respectively;the Cmax of ciprofloxacin(Metabolite)was 0.72,0.89 and 1.21 mg·kg^(-1),respectively;the enrofloxacin tmax was 1,4 and 4 h,respectively;the ciprofloxacin tmax was 4,12 and 4 h,respec-tively;the enrofloxacin t1/2z was 38.22,52.44 and 62.40 h,respectively;the ciprofloxacin t1/2z was 66.23,33.11 and 27.06 h,respectively;the theoretical drug rest period was 16.94,16.79 and 17.99 d,respectively.The results indicate that for all tissues,20 mg·kg^(-1) of enrofloxacin mixture with the Cmax/MIC over 10 is suitable for the treatment of B.areolate disease caused by Vibrio harveyi(MIC,0.45 mg·L^(-1)).It is recommended that the drug rest period should not be less than 440.76℃·d and the dosing should be once every 2 d,a total of 3 times.
作者
邓东
文永森
刘晓坤
吕文刚
陈景雷
陈景步
DENG Dong;WEN Yongsen;LIU Xiaokun;LYU Wengang;CHEN Jinglei;CHEN Jingbu(Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China;Invertebrate Science and Technology Innovation Center,Zhanjiang 524088,China;Hainan Kangguan Biotechnology Co.,Ltd.,Wenchang 571300,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2024年第6期176-186,共11页
South China Fisheries Science
基金
广东省现代化海洋牧场适养品种核心技术攻关项目(2024-MRB-00-001)
国家重点研发计划“蓝色粮仓”科技创新专项(2018YFD0901401)
国家自然科学基金青年科学基金项目(31702325)。
