摘要
目的分析结直肠癌(CRC)组织中lncRNA RPPH1、前梯度蛋白3(AGR3)的表达情况及其临床意义。方法招募2017年1月至2020年1月西电集团医院收治的CRC患者98例。于术中采集CRC组织及癌旁组织(距离癌组织边缘3 cm以上,病理检查未发现癌细胞)。采用实时定量聚合酶链反应(PCR)检测lncRNA RPPH1表达水平,采用免疫组化染色检测AGR3表达情况。分析CRC组织lncRNA RPPH1、AGR3表达与患者临床病理特征及生存预后的关联性。结果CRC组织lncRNA RPPH1表达水平显著高于癌旁组织(P<0.05),AGR3阳性率显著高于癌旁组织[65.31%(64/98)vs 10.20%(10/98),P<0.001]。CRC组织lncRNA RPPH1、AGR3表达与肿瘤TNM分期、肿瘤分化程度以及淋巴结转移情况均存在显著关联(P<0.05)。lcRNA RPPH1低表达组的生存预后显著优于高表达组(log-rank检验:χ^(2)=9.222,P=0.002)。AGR3阴性组的生存预后显著优于阳性组(log-rank检验:χ^(2)=8.090,P=0.005)。多因素Cox回归分析结果显示,发生淋巴结转移、肿瘤TNM分期为Ⅲ期、lncRNA RPPH1高表达、AGR3阳性是CRC患者生存预后不佳的独立危险因素(P<0.05)。结论CRC组织中lncRNA RPPH1表达水平升高、AGR3阳性表达与患者的不良临床病理特征及不良预后有关。
Objective To analyze the expressions of long non-coding RNA(lncRNA)ribonuclease P RNA component H1(RPPH1)and anterior gradient protein 3(AGR3)in colorectal cancer(CRC)tissues and their clinical significance.Methods A total of 98 patients with CRC who were admitted to XD Group Hospital from January 2017 to January 2020 were recruited.During the operation,CRC tissues and their paracancer tissues(at a distance of more than 3 cm from the edge of the cancer tissue,and no cancer cells being found by pathological examination)were collected.The expression levels of lncRNA RPPH1 were detected by using real-time quantitative polymerase chain reaction(PCR).The expression of AGR3 was detected by using immunohistochemical staining.The correlations of the expressions of lncRNA RPPH1 and AGR3 in the CRC tissues with the patients′clinicopathological features and survival prognosis were analyzed.Results The expression level of lncRNA RPPH1 in the CRC tissues was significantly higher than that in the paracancer tissues(P<0.05),and the positive rate of AGR3 in the CRC tissues was significantly higher than that in the paracancer tissues[65.31%(64/98)vs 10.20%(10/98),P<0.001].The expressions of lncRNA RPPH1 and AGR3 in the CRC tissues were significantly correlated with tumor TNM stage,tumor differentiation degree and lymph node metastasis(P<0.05).The survival prognosis of the low lcRNA RPPH1 expression group was significantly better than that of the high lcRNA RPPH1 expression group(log-rank test:χ^(2)=9.222,P=0.002).The survival prognosis of the AGR3-negative group was significantly better than that of the AGR3-positive group(log-rank test:χ^(2)=8.090,P=0.005).The results of multivariate Cox regression analysis showed that lymph node metastasis,tumor TNM stageⅢ,high expression of lncRNA RPPH1 and AGR3 positive were independent risk factors of poor survival prognosis for the CRC patients(P<0.05).Conclusion The increased expression level of lncRNA RPPH1 and positive expression of AGR3 in CRC tissues are associated with adverse c
作者
平胜
牟亚刚
程璇
李祥
庄云龙
PING Sheng;MOU Yagang;CHENG Xuan;LI Xiang;ZHUANG Yunlong(Department of General Surgery,XD Group Hospital,Xi′an 710082,China)
出处
《中国临床新医学》
2024年第11期1244-1249,共6页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
陕西省科技计划项目(编号:2021JQ⁃926)。
关键词
结直肠癌
lncRNA
RPPH1
前梯度蛋白3
生存预后
Colorectal cancer(CRC)
Long non-coding RNA(lncRNA)ribonuclease P RNA component H1(RPPH1)
Anterior gradient protein 3(AGR3)
Survival prognosis
