摘要
为增强酸性含氮废水生物脱氮效果和提高水处理微生物对酸性水质条件的适应性,采用“自下而上”的脱氮菌群构建策略,利用统计分析和部分因子实验筛选核心菌株,并围绕核心菌株理性设计构建了一组反硝化复合菌群S119-S136-L22,其在p H值为4.0,NO_(3)^(-)-N初始质量浓度200mg/L的条件下,36h硝氮去除率为97.32%.通过全基因组测序并结合各菌株与氮代谢、反硝化作用相关基因分析,推测菌群中菌株S136和L22主要负责将NO_(3)^(-)-N还原成NO_(2)^(-)-N,S119负责将NO_(2)^(-)-N还原为N_(2).序批式反应器(SBR)研究显示,实验组较空白组NO_(3)^(-)-N、TN的去除率分别提高28.99%、28.67%.高通量测序与系统微生物群落互作网络分析表明,相较于空白组,除了Enterobacter、Alcaligenes和Klebsiella,实验组还出现了2个差异功能菌属Cloacibacterium、Serratia.实验组呈现出更为复杂多样的正相关路径,不仅复合菌群中各菌属间呈协同促进作用,Serratia与Acinetobacter、Achromobacter,Cloacibacteriu与Macellibacteroides和Pleomorphomonas也呈共线性关系.出水TN、NO_(3)^(-)-N与微生物菌群互作网络分析,Cloacibacterium主要驱动TN、NO_(3)^(-)-N的去除,Pleomorphomonas、Microbacterium、Propionicicella在TN、NO_(3)^(-)-N的去除过程中也发挥了重要作用.
To enhance the biological nitrogen removal efficiency of acidic nitrogen-containing wastewater and improve the adaptability of water treatment microorganisms to acidic water conditions,a"bottom-up"strategy for constructing a nitrogen removal bacterial consortium was adopted.Core strains were selected through statistical analysis and partial factorial experiments,and a group of denitrifying composite bacteria,S119-S136-L22,was rationally designed and constructed around these core strains.Under conditions of p H 4.0 and an initial nitrate nitrogen (NO_(3)~--N) concentration of 200mg/L,the nitrate removal rate reached 97.32%after 36hours.Through whole-genome sequencing combined with analysis of genes related to nitrogen metabolism and denitrification in each strain,it was speculated that strains S136 and L22 in the consortium are mainly responsible for reducing NO_(3)~--N to nitrite nitrogen (NO_(2)~--N),while S119was responsible for reducing NO_(2)~--N to nitrogen gas (N_(2)).Sequencing batch reactor (SBR) studies showed that the removal rates of NO_(3)~--N and total nitrogen (TN) in the experimental group were increased by 28.99%and 28.67%,respectively,compared to the control group.High-throughput sequencing and analysis of the microbial community interaction network in the system indicated that,compared to the control group,two additional functionally distinct bacterial genera,Cloacibacterium and Serratia,emerged in the experimental group,along with Enterobacter,Alcaligenes,and Klebsiella.The experimental group exhibited a more complex and diverse network of positive correlation pathways,not only showing synergistic interactions between the bacterial genera within the composite consortium but also collinearity between Serratia,Acinetobacter and Achromobacter,as well as between Cloacibacterium,Macellibacteroides and Pleomorphomonas.Analysis of the interaction network between effluent TN,NO_(3)~--N,and the microbial community revealed that Cloacibacterium primarily drives the removal of TN and NO_(3)~--N,while Pleo
作者
任晓兰
牛浩英
袁进
端允
范晓军
REN Xiao-lan;NIU Hao-ying;YUAN Jin;DUAN Yun;FAN Xiao-jun(College of the Environment and Ecology,Taiyuan University of Technology,Jinzhong 030600,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第12期6708-6720,共13页
China Environmental Science
基金
国家自然科学基金资助项目(52170045)
山西省重点研发计划资助项目(202102130501008)。
关键词
酸性含氮废水
复合菌群
脱氮
全基因组测序
互作网络
acidic nitrogen-containing wastewater
bacterial consortium
nitrogen removal
whole-genome sequencing
interaction network
