摘要
目的通过超高效液相色谱串联飞行时间质谱(UPLC-TOF-MS)技术,探究益经汤颗粒对卵巢早衰小鼠血清代谢组学的影响。方法将实验小鼠随机分为模型组、阳性对照组、益经汤低、高剂量组和空白组,每组6只。使用环磷酰胺诱导卵巢早衰小鼠模型,灌胃给药进行干预,每日1次,共30 d。通过UPLC-TOF-MS技术,分析小鼠血清样本中内源代谢物变化,应用主成分分析(PCA)以及正交偏最小二乘判别分析(OPLS-DA)等方法,通过变量投影重要性值(VIP)和t检验结果对潜在差异代谢物进行筛选,并借助MetaboAnalyst平台进行代谢通路富集分析。结果代谢组学分析得到模型组较空白组相比存在潜在差异代谢物74个,益经汤高剂量组较模型组相比共有潜在差异代谢物130个。经再次筛选鉴定得到25个与益经汤治疗主要相关的差异代谢物,共富集分析得到KEGG通路11条,其中主要代谢通路有组氨酸代谢、柠檬酸循环、氨基糖和核苷酸糖代谢通路、果糖和甘露糖代谢以及赖氨酸降解等。结论益经汤颗粒对环磷酰胺诱导的卵巢早衰小鼠发挥治疗作用,其机制可能与改善血清中差异代谢物水平以及调节组氨酸、柠檬酸循环、果糖和甘露糖、赖氨酸代谢有关。
Objective To investigate the effect of Yijing Decoction granules on serum metabolomics in mice with premature ovarian failure by ultra-performance liquid chromatography tandem time-of-flight mass spectrometry(UPLC-TOF-MS).Methods The experimental mice were randomly divided into the model group,the positive control group,the low and high doses of Yijing Decoction granules groups and blank group,6 mice in each group.The mouse model of premature ovarian failure was induced using cyclophosphamide,and the drug was administered by gavage for intervention once a day for 30 days.The changes of endogenous metabolites in mouse serum samples were analyzed by UPLC-TOF-MS.The potential differences of metabolites were screened by the value of the projected importance of the variables(VIP)and the results of t-test by applying the methods of principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).The metabolic pathway enrichment analysis was performed using the MetaboAnalyst platform.Results Metabolomics analysis showed that there were 74 potentially different metabolites in the model group compared with the blank group,and there were 130 potentially different metabolites in high-dose Yijing Decoction granules group compared with the model group.After re-screening,25 different metabolites were identified,and 11 KEGG pathways were enriched,including histidine metabolism,citric acid cycle,aminosugar and nucleotide sugar metabolism,fructose and mannose metabolism,and lysine degradation,etc.Conclusion The mechanism of the therapeutic effect exerted by Yijing Decoction granules on cyclophosphamide-induced POF mice may be related to the improvement of the levels of differential metabolites in serum,as well as the modulation of histidine,citric acid cycle,fructose and mannose,and lysine metabolism.
作者
陈烁
牛逸琳
王峰
尹永芹
何冬梅
崔红花
CHEN Shuo;NIU Yilin;WANG Feng;YIN Yongqin;HE Dongmei;CUI Honghua(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;Obstetrics and Gynecology Department,Huizhou Hospital of Traditional Chinese Medicine,Huizhou 516001,China)
出处
《广东药科大学学报》
CAS
2024年第6期31-39,共9页
Journal of Guangdong Pharmaceutical University
基金
广东省惠州市中医医院博士工作站科研项目(BSGZZ03)
广东省中医药局科研项目(20242050)
广东省自然科学基金项目(2021A1515011529)。
