摘要
植物分类学实验是高校植物学课程的重要组成部分,是学习植物分类学重要的实践环节。在传统教学中,教师多借助校园植物、标本馆、公园和自然保护区等场地,帮助学生快速建立植物分类的基本体系。随着生物技术快速发展,人们开始利用植物DNA中携带的遗传信息来鉴定植物种类,植物DNA条形码技术逐渐成为新兴的研究领域和植物鉴定手段。因此,在高校的植物分类学实验中引入植物DNA条形码技术,不仅可以加强学生对植物分类学知识的理解和掌握,还可以提高其分子生物学实验技能和科学实验素养。以校园常见裸子植物为例,利用ITS2和rbcL序列对其进行DNA条形码研究,探讨这些类群的亲缘关系,为准确鉴定提供分子证据。同时,提出基于植物DNA条形码技术在高校植物分类学实验中的教学设计,让学生从形态和分子角度理解常见校园植物的特征和演化关系。
Plant taxonomy experiments have always been a crucial component of university botany courses serving as a sgnificant practical link in the pursuit of mastering plant taxonomy.In traditional teaching methods teachers usually establish a fundamental system of plant classification for students depend on campus plants specimen halls parks and nature reserves.With the rapid advancement of biotechnology however people have begun to utilize the genetic information carried within plant DNA to identify plant species.The emerging field of plant DNA barcoding technology has gradually become both a thriving research area and an efficient plant identification method.Therefore incorporating this technology into plant taxonomy experiments in universities can not only enhance studentscomprehension and grasp of plant taxonomy knowledge but also improve their molecular biology experimental skills and scientific experimental literacy.In this study common gymnosperms found on campus were taken as a case in point to study their DNA barcodes using ITS2 and rbcL sequences,to explore the phylogenetic relationships of these groups,and to provide molecular evidence for their accurate identification as well.Meanwhile,a teaching design was proposed based on plant DNA barcode technology in university plant taxonomy experiments that allows students to comprehend the characteristics and evolutionary relationships of common campus plants from both a morphological and molecular perspective.
作者
周亚东
杨过
管毕财
ZHOU Yadong;YANG Guo;GUAN Bicai(School of Life Sciences,Nanchang University,Nanchang 330031,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2024年第6期116-120,125,共6页
Journal of Biology
基金
教育部产学合作协同育人项目“植物学实验虚拟仿真课程建设”(202102315006)。
