摘要
目的探讨急性缺血性脑血管病患者血清中miR-342-3p和miR-30a-5p的表达及意义。方法选取2022年3月至2023年3月我院收治的急性缺血性脑血管病患者195例作为急性缺血性脑血管病组,另选取同期103例健康体检者作为对照组。采用实时荧光定量PCR法检测血清miR-342-3p、miR-30a-5p表达水平;相关性行Spearman法分析诊断价值采用受试者工作特征曲线评估。结果与对照组比较,急性缺血性脑血管病组患者血清的miR-342-3p水平显著升高,miR-30a-5p水平均显著降低(P<0.05);急性缺血性脑血管病组与对照组的低密度脂蛋白胆固醇(LDL-C)、收缩压、舒张压相比,差异有统计学意义(P<0.05);两组高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)、总胆固醇(TC)等因素相比,差异无统计学意义(P>0.05)。不同神经功能缺失患者血清miR-342-3p、miR-30a-5p、NIHSS评分差异均有统计学意义(P<0.05),且重度组患者血清miR-342-3p水平、NIHSS评分高于轻度组和中度组,miR-30a-5p低于轻度组和中度组(P<0.05)。血清miR-342-3p水平与NIHSS评分呈正相关(r=0.601,P<0.05);血清miR-30a-5p水平与NIHSS评分呈负相关(r=-0.695,P<0.05)。血清miR-342-3p和miR-30a-5p水平及其联合在诊断急性缺血性脑血管病患者重度神经功能缺失中的曲线下面积(AUC)分别为0.800、0.817、0.877,敏感度分别为68.12%、73.91%、86.96%,两者联合诊断价值高于单独诊断(Z二者联合-miR-342-3p=2.081,P=0.038;Z二者联合-miR-30a-5p=2.026,P=0.043)。结论急性缺血性脑血管病患者血清miR-342-3p血清表达水平升高,miR-30a-5p表达水平降低,二者对急性缺血性脑血管病患者神经功能缺失程度具有一定的诊断价值。
Objective To investigate the expression and significance of microRNA-342-3p(miR-342-3p)and microRNA-30a-5p(miR-30a-5p)in the serum of patients with acute ischemic cerebrovascular disease.Methods From March 2022 to March 2023,total 195 patients with acute ischemic cerebrovascular disease accepted by our hospital were regarded as acute ischemic cerebrovascular disease group,and 103 healthy individuals were as reference group.Real time fluorescence quantitative PCR was used to detect the expression levels of serum miR-342-3p and miR-30a-5p.The Spearman method is used to analyze the diagnostic value of correlation analysis,and the subject work characteristic curve is used to evaluate it.Results Compared with reference group,acute ischemic cerebrovascular disease group showed a obvious increase in serum miR-342-3p and a obvious decrease in miR-30a-5p level(P<0.05).There was a statistically obvious difference in low-density lipoprotein cholesterol(LDL-C),systolic blood pressure,and diastolic blood pressure between acute ischemic cerebrovascular disease group and reference group(P<0.05).There was no obvious difference in factors such as high-density lipoprotein cholesterol(HDL-C),triglycerides(TG),and total cholesterol(TC)(P>0.05).There were obvious differences in serum miR-342-3p,miR-30a-5p,and NIHSS scores among patients with different neurological deficits(P<0.05),moreover,serum miR-342-3p and NIHSS score in severe group were higher than those in the mild and moderate groups,while miR-30a-5p level was lower than that in the mild and moderate groups(P<0.05).Serum miR-342-3p was positively correlated with NIHSS score(r=0.601,P<0.05).Serum miR-30a-5p level was negatively correlated with the NIHSS score(r=-0.695,P<0.05).The area under the curve(AUC)of serum miR-342-3p and miR-30a-5p and in combination for diagnosing severe neurological deficits in patients with acute ischemic cerebrovascular disease was 0.800,0.817,and 0.877,respectively,with sensitivity of 68.12%,73.91%,and 86.96%,respectively.The combined diagnostic va
作者
王瑶
曹娜
肖兵
Wang Yao;Cao Na;Xiao Bing(Department of Neurology,Chengdu Third People's Hospital,Chengdu,Sichuan 610031,China)
出处
《四川医学》
CAS
2024年第11期1247-1252,共6页
Sichuan Medical Journal
