摘要
为探究南极磷虾油(KO)对帕金森病(PD)小鼠脑黑质多巴胺能神经元及肠道菌群的影响,采用腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)的方法构建PD亚急性模型,将小鼠分为对照组(Control)、PD模型组(PD)、KO干预组(KO_PD)和KO对照组(KO),采用TH免疫组化技术评估KO对PD小鼠脑黑质多巴胺能神经元及其神经纤维的保护作用,并结合小鼠结肠HE染色及小鼠粪便16S rDNA高通量测序技术,研究KO对PD小鼠肠道结构及肠道微生态的影响。结果显示:与PD组相比,KO_PD组小鼠脑黑质TH阳性细胞个数显著增加约5倍,上皮杯状细胞数量增加约2倍,结肠肠腺稀疏等现象均有所改善。PD组小鼠肠道菌群紊乱,而KO干预显著增加了拟杆菌门和杜氏杆菌属、Muribaculaceae_unclassified、链球菌属等的相对丰度,下调厚壁菌门和另枝菌属、梭菌目未知菌属、螺杆菌属等的相对丰度。结论:KO可有效改善MPTP诱导的小鼠黑质区多巴胺能神经元丢失和结肠组织结构的改变,并有助于在各分类水平上维持小鼠肠道菌群稳态,且该作用可能与影响肠道菌群执行糖代谢、氨基酸代谢、脂质代谢等生命功能相关。
To investigate the effect of Antarctic krill oil(KO)on dopaminergic neurons and gut microbiome in Parkinson's disease(PD)mice,the method of intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)were used to construct PD subacute model in mice.The mice were divided into Control group,PD group,KO_PD group and KO group.The immunohistochemical technique of TH was used to evaluate the protective effect of Antarctic krill oil on dopaminergic neurons and nerve fibers in PD mice.Combined with HE staining of colon and 16S rDNA high-throughput sequencing of feces,the effect of KO on the intestinal structure and intestinal microecology of PD mice was studied.The results indicated that,compared with the PD group,the number of TH-positive cells in the substantia nigra of the brain was significantly increased by about 5-fold,the number of epithelial cuprocytes was increased by about 2-fold,and the phenomena such as thinning of colonic intestinal glands were improved in the KO_PD group of mice.The gut microbiota of PD group mice was disrupted,while KO intervention significantly increased the relative abundance of Bacteroidetes phylum,Dubosiella genus,and Muribaculaceae_unclassified genus,Streptococcus genus,etc.,and significantly downregulated the relative abundance of Firmicutes phylum,Alistipes genus,Clostridiales_unclassified genus and Helicobacter genus,etc.To sum up,oral administration of KO can effectively improve the loss of dopaminergic neurons in the substantia nigra and structural changes in the colon tissue of mice induced by MPTP injection,and maintain the homeostasis of mice gut microbiota at various levels.This effect may be related to the regulation of glucose metabolism,amino acid metabolism,and lipid metabolism,etc.,in gut microbiota.
作者
郑慧敏
卢蔚波
葛丽君
赫晓菡
薛静
Zheng Huimin;Lu Weibo;Ge Lijun;He Xiaohan;Xue Jing(Institute of Sea Food,Zhejiang Gongshang University,Hangzhou 310012;State Key Laboratory of Aquatic Products Processing of Zhejiang Province,Zhejiang Gongshang University,Hangzhou 310012)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第10期134-144,共11页
Journal of Chinese Institute Of Food Science and Technology
基金
国家重点研发计划项目(2018YFC0311204)。
