摘要
目的检测肺腺癌患者癌组织中扩展突触结合蛋白3(ESYT3)和Ⅲ型纤连蛋白结构域包含蛋白1(FNDC1)的表达,并分析两者与肿瘤进展的关系。方法纳入2019-06-01-2022-06-01河南省胸科医院收治的137例肺腺癌患者病理组织标本。患者男96例,女41例;年龄43~76岁,平均年龄(65.87±8.14)岁。应用免疫组织化学染色法检测癌组织、癌旁组织(距离癌组织≥3 cm)ESYT3和FNDC1蛋白的阳性表达,分析其与临床病理特征的关系。随访截至2023-10-01,比较不同进展状态患者癌组织ESYT3和FNDC1阳性表达情况。采用χ^(2)检验分析ESYT3和FNDC1阳性表达与肺腺癌临床病理特征的关系,采用多因素Cox比例风险回归模型分析肺腺癌进展的影响因素,绘制Kaplan-Meier曲线分析癌组织ESYT3和FNDC1蛋白表达与肺腺癌进展的关系。结果肺腺癌组织和癌旁组织中,ESYT3阳性表达率分别为33.58%(46/137)和56.20%(77/137),FNDC1阳性表达率分别为43.07%(59/137)和21.90%(30/137),差异均有统计学意义,χ^(2)值分别为14.177和13.995,均P<0.001。有吸烟史、TNMⅢ期、有淋巴结转移、未/低分化、胸膜侵袭患者,癌组织ESYT3阳性表达率分别低于无吸烟史(χ^(2)=7.344,P=0.007)、TNMⅠ/Ⅱ期(χ^(2)=7.766,P=0.005)、无淋巴结转移(χ^(2)=7.161,P=0.007)、中/高分化(χ^(2)=6.742,P=0.009)、无胸膜侵袭(χ^(2)=8.408,P=0.004)患者,癌组织FNDC1阳性表达率分别高于无吸烟史(χ^(2)=9.352,P=0.002)、TNMⅠ/Ⅱ期(χ^(2)=6.691,P=0.010)、无淋巴结转移(χ^(2)=4.258,P=0.039)、中/高分化(χ^(2)=7.421,P=0.006)、无胸膜侵袭(χ^(2)=7.738,P=0.005)患者。随访时间16~52个月,中位随访时间37(18,48)个月,124例患者进展率50.81%(63/124)。多因素Cox比例风险回归模型分析显示,吸烟史(HR=3.254,95%CI:1.339~7.908)、TNMⅢ期(HR=2.125,95%CI:1.279~3.531)、未/低分化(HR=2.032,95%CI:1.230~3.356)、癌组织FNDC1表达(HR=2.980,95%CI:1.570~5.657)是肺腺癌进展的危险因素,癌组织ESYT3表达(HR
Objective To detect the expression of extended synaptic binding protein 3(ESYT3)and typeⅢfibronectin domain-containing protein 1(FNDC1)in lung adenocarcinoma patients,and to analyze their relationship with tumor progression.Methods Pathological tissue specimens of 137patients with lung adenocarcinoma admitted to Henan Chest Hospital from June 1,2019to June 1,2022were included.There were 96males and 41females.The average age was(65.87±8.14)years,ranging from 43to 76years.Immunohistochemical staining was used to detect the positive expression of ESYT3and FNDC1proteins in cancer tissues and adjacent tissues(≥3cm from cancer tissues),and to analyze the relationship between the positive expression of ESYT3and FNDC1and clinicopathological features.The patients were followed up until October 1,2023to compare the positive expression of ESYT3and FNDC1in cancer tissues of patients with different progression.χ^(2) test was used to analyze the relationship between ESYT3and FNDC1positive expression and clinicopathological features of lung adenocarcinoma.Multi-factor Cox proportional risk regression model was used to analyze the influencing factors of lung adenocarcinoma progression,and Kaplan-Meier curve was plotted to analyze the relationship between ESYT3and FNDC1protein expression in cancer tissue and lung adenocarcinoma progression.Results The positive expression rates of ESYT3and FNDC1were 33.58%(46/137)and 56.20%(77/137)respectively in lung adenocarcinoma tissues and adjacent tissues,and the positive expression rates of FNDC1were 43.07%(59/137)and 21.90%(30/137),respectively,with statistically significant differences,χ^(2) value being 14.177and 13.995,respectively,both P<0.001.The ESYT3positive expression rate of patients with smoking history,TNM stageⅢ,lymph node metastasis,undifferentiated/poorly differentiated,and pleural invasion was lower than that of patients with no smoking history(χ^(2)=7.344,P=0.007),TNM stageⅠ/Ⅱ(χ^(2)=7.766,P=0.005),and no lymph node metastasis(χ^(2)=7.161,P=0.007),moderately/h
作者
马小杰
董庆芬
刘娟
李希婷
祁敏现
MA Xiaojie;DONG Qingfen;LIU Juan;LI Xiting;QI Minxian(Department of Pathology,Henan Chest Hospital,Zhengzhou,Henan 450008,China;Department of Oncology,Xinwen Central Hospital of Shandong Yiyang Health Group,Taian,Shandong 271200,China)
出处
《中华肿瘤防治杂志》
CAS
北大核心
2024年第19期1170-1176,共7页
Chinese Journal of Cancer Prevention and Treatment
关键词
肺腺癌
扩展突触结合蛋白3
Ⅲ型纤连蛋白结构域包含蛋白1
肿瘤进展
lung adenocarcinoma
extended synaptotagmin 3(ESYT3)
fibronectin typeⅢdomain-containing protein 1(FNDC1)
tumor progression
