摘要
介绍了减肥类保健食品中酚酞、西布曲明基体标准物质的研制过程。采用阴性加标的方式,向保健食品模拟基质中添加酚酞、西布曲明标准溶液,混匀后分装制成保健食品标准样品。采用高效液相色谱-三重四级杆串联质谱法对其中酚酞、西布曲明的含量进行检测,并对样品中酚酞、西布曲明含量的均匀性和稳定性进行评价,5家实验室协作定值,评估定值的不确定度。研制的减肥类保健食品中酚酞、西布曲明基体标准物质定值结果分别为(19.8±2.95)、(18.8±3.03)mg/kg,均匀性和稳定性良好。研制流程和结果符合基体标准物质定值的要求,该标准物质可用于相关基质样品检测的实验室内部质量控制。
The development process of phenolphthalein and sibutramine matrix reference materials of weight loss health foods was introduced.The standard solutions of phenolphthalein and sibutramine were added to the simulated matrix of health food by negative addition method.After mixing,the standard sample of health food was prepared.The contents of phenolphthalein and sibutramine were detected by high performance liquid chromatography-triple quadrupole tandem mass spectrometry.The uniformity and stability of the contents of phenolphthalein and sibutramine in the samples were evaluated.Five laboratories cooperated to set the values and evaluated the uncertainty of the values.The certified values of phenolphthalein and sibutramine in the weight loss health food were(19.8±2.95)mg/kg and(18.8±3.03)mg/kg,respectively.The uniformity and stability were good.The development process and results meet the requirements of the certified value of the matrix reference material.The reference materials can be used for the internal quality control of the laboratory for the detection of related matrix samples.
作者
李梦怡
王宏伟
王玉梅
董喆
曹进
LI Mengyi;WANG Hongwei;WANG Yumei;DONG Zhe;CAO Jin(National Institutes for Food and Drug Control,Key Laboratory of Food Quality and Safety for State Market Regulation,Beijing100050,China;Center for Food Evaluation,State Administration for Market Regulation,Beijing 100070,China)
出处
《化学分析计量》
CAS
2024年第11期1-5,12,共6页
Chemical Analysis And Meterage
基金
中华人民共和国科学技术部“食品安全关键技术研发”重点专项(2017YFC1601304)。
关键词
标准物质制备
减肥类保健食品
高效液相色谱-三重四级杆串联质谱法
酚酞
西布曲明
不确定度
development of standard materials
weight loss health food
high performance liquid chromatography-triple quadrupole tandem mass spectrometry
phenolphthalein
sibutramine
uncertainty
