摘要
由于季节更替或天气骤变,水温骤降时有发生,养殖虾类很容易受到低温胁迫,sRNA分析可为其抗逆分子机制的探索提供新的技术手段。研究了低温胁迫对脊尾白虾(Exopalaemon carinicauda)微小核糖核酸Micro RNA(mi RNA)及其对应靶基因表达的影响,筛选可能与低温调控关联的mi RNA,对体质量(1.27±0.15)g的脊尾白虾进行低温(0℃)处理4 h,对照组水温维持在25℃,sRNA测序得到20.45M bp过滤序列。通过比对获得55个保守的miRNA和9个非保守的miRNA。定量低温胁迫脊尾白虾miRNA表达丰度筛出11个显著上调的miRNA,其中dme-miR-100-3p miRNA上调幅度最大,其次为dme-miR-2491-5p miRNA。599个靶基因差异表达显著,GO分类发现差异表达miRNA对刺激反应、生物调节、粘附、催化活性和代谢过程等靶基因调控显著;COG分类发现差异表达miRNA对蛋白周转和伴侣蛋白、碳水化合物和氨基酸运输和代谢等靶基因调控显著;KEGG富集分析发现靶基因二氢硫辛酯脱氢酶和乳酸脱氢酶在糖酵解/糖异生、丙酮酸代谢、氨基酸代谢和降解等通路得到富集,而靶基因钙/钙调蛋白依赖性蛋白激酶在Wnt信号通路、钙信号通路和ErbB信号通路等得到富集。结果表明,低温环境下差异表达miRNA和相关靶基因在脊尾白虾的跨膜信号转导、蛋白质代谢和碳水化合物代谢等环节的调控中发挥着重要作用,因而推测低温通过脊尾白虾miRNA上调细胞糖酵解和三羧酸循环途径促进体内蛋白质和碳水化合物等底物分解供能来适应逆境。研究结果可为进一步探究脊尾白虾低温胁迫下miRNA差异表达及其功能奠定基础。
Studying the effects of low temperature stress on the expression of microRNA(miRNA)and its corresponding target genes in Exopalemon carinicauda,and screening for miRNAs that may be associated with low temperature regulation will help reveal the role of miRNAs in the mechanism of low temperature tolerance.E.carinicauda with a body weight of(1.27±0.15)g was subjected to low temperature(0℃)treatment for 4 h,the control group was maintained at 25℃,and a 20.45M bp filtered sequence was obtained through sRNA sequencing.Through comparison,55 conserved miRNAs and 9 non conserved miRNAs were obtained.Quantitative analysis of miRNA expression abundances in E.carinicauda under low temperature stress revealed 11 significantly up-regulated miRNAs,of which dme-miR-100-3p was the most up-regulated miRNA after low temperature stress,and its corresponding target genes were mainly potassium channel and serine protease inhibitor.The miRNA with the second highest up-regulation after low temperature stress was dme-miR-2491-5p,which corresponded to target genes such as acetyllactate synthase and sodium/glucose co transporter protein.The miRNA with the third highest up-regulation after low temperature stress was rno miR-675-3p,and the corresponding target genes mainly included amino acid transporters and alkaline phosphatase.In addition,miRNA-hsa-miR-671-3p was significantly up-regulated after low temperature stress,and its corresponding target genes were mainly lactate dehydrogenase and glucosidase.In summary,E.carinicauda responded positively to temperature changes after low temperature stress by activating signaling pathways such as carbohydrate and protein metabolism,mobilizing various glucose and amino acid metabolism in the body to provide energy to resist low temperature stress and maintain body homeostasis.599 target genes showed significant differential expression.According to GO classification and enrichment analysis,it was found that the differentially expressed miRNAs were significantly regulated by target genes such a
作者
陈吉圣
卢雪旎
王兴强
曹梅
李永
秦传新
CHEN Jisheng;LU Xueni;WANG Xingqiang;CAO Mei;LI Yong;QIN Chuanxin(College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang Jiangsu 222005,China;Lianyungang Qiaohai Fishery Technology Co.Ltd.,Lianyungang Jiangsu,222045,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)
出处
《海洋渔业》
CSCD
北大核心
2024年第5期616-627,共12页
Marine Fisheries
基金
江苏省政策引导类计划苏北科技专项(SZ-LYG202038)。
