摘要
目的探究外源性趋化因子配体12(CXCL12)通过激活趋化因子受体4(CXCR4)/蛋白激酶B(AKT)信号通路对肺癌细胞迁移的作用。方法用外源性CXCL12处理肺癌细胞,通过Western blot实验对自噬相关蛋白LC3B以及CXCR4/AKT信号通路蛋白进行检测,通过划痕实验对细胞的迁移进行检测。结果与对照组相比较,加入外源性CXCL12组细胞的迁移能力增强(t=3.949,P<0.05),自噬相关蛋白LC3BⅡ的表达水平显著增高(t=3.051,P<0.05),CXCR4的表达和AKT的磷酸化水平也明显增高(t=2.974、4.307,P<0.05)。结论CXCL12可能通过CXCR4/AKT信号通路诱导自噬进而促进肺癌细胞的迁移。
Objective To investigate the effect of exogenous CXCL12 on the migration of lung cancer cells by activating the CXCR4/AKT signaling pathway.Methods Lung cancer cells were treated by exogenous CXCL12.Western blot was used to measure the autophagy-related protein microtubule-associated protein light chain 3BⅡ(LC3BⅡ)and the proteins associated with the CXCR4/AKT signaling pathway,and wound healing assay was used to observe cell migration.Results Compared with the control group,the group with the addition of exogenous CXCL12 had significant increases in the migration ability of cells(t=3.949,P<0.05)and the expression of the autophagy-related protein LC3BⅡ(t=3.051,P<0.05),as well as significant increases in the expression of CXCR4 and the phosphorylation level of AKT(t=2.974,4.307,P<0.05).Conclusion CXCL12 may promote the migration of lung cancer cells by inducing autophagy via the CXCR4/AKT signaling pathway.
作者
张一帆
万里新
李慧
孙晓
ZHANG Yifan;WAN Lixin;LI Hui;SUN Xiao(Department of Pulmonary Oncology,Nanyang Central Hospital,Nanyang 47300,China)
出处
《青岛大学学报（医学版）》
CAS
2024年第5期669-672,共4页
Journal of Qingdao University(Medical Sciences)
基金
河南省医学科技攻关计划省部共建项目(SBGJ-202102220)。
