摘要
目的观察肝豆汤对Wilson病(Wilson’s disease,WD)模型小鼠肝脏脂质代谢的调控作用并探究其机制。方法以10只DL小鼠作为正常组,将20只TX小鼠随机分为模型组、肝豆汤组,每组10只。模型组、正常组小鼠均予以生理盐水25 mL/(kg·d)灌胃4周,肝豆汤组小鼠予以肝豆汤18.5 g/(kg·d)灌胃4周。使用电感耦合等离子体质谱仪检测小鼠肝脏铜含量,采用生化分析仪检测小鼠血清总胆固醇(total cholesterol,TC)、三酰甘油(triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸转移酶(aspartate aminotransferase,AST)水平,检测肝组织丙二醛(malondialdehyde,MDA)含量以评价肝脏中脂质过氧化程度,采用油红O(oil red O,ORO)染色检测肝脏脂质沉积情况,采用RT-qPCR检测小鼠肝脏组织脂质代谢相关蛋白[脂肪细胞三酰甘油水解酶(adipocyte TG hydrolase,ATGL)、脂肪酸合酶(fatty acid synthase,FASN)、乙酰辅酶A羧化酶1(acetyl-CoA carboxylase 1,ACC1)、固醇调节元件结合蛋白1c(sterol regulatory element-binding protein-1c,SREBP-1c)]及过氧化物酶体增殖物激活受体γ(peroxisome proliferator activated receptors gamma,PPARγ)、白细胞分化原36(cluster of differentiation 36,CD36)mRNA表达水平,采用Western blot法检测肝脏PPARγ、CD36蛋白表达水平。结果与正常组比较,模型组小鼠肝脏中铜含量显著升高(P<0.05);肝豆汤组小鼠肝脏中铜含量较模型组显著降低(P<0.05)。模型组小鼠血清TG、TC、LDL水平均较正常组显著降低(P<0.05),肝豆汤组小鼠血清TG、TC、LDL水平较模型组显著升高(P<0.05)。模型组小鼠肝脏MDA含量较正常组显著升高(P<0.05),肝豆汤组小鼠MDA含量较模型组显著降低(P<0.05)。ORO染色结果显示,模型组小鼠肝脏出现广泛脂质沉积,肝豆汤组小鼠肝脏ORO脂滴面积较模型组显著降低(P<0.05)。与正常组比较,模型组小鼠�
Objective To investigate the regulatory effect of Gandou Decoction on liver lipid metabolism in Wilson s disease(WD)mice and its mechanism.Methods A total of 10 DL mice were used as normal group,and 20 TX mice were randomly divided into model group and Gandou Decoction group,with 10 mice in each group.The mice in the model group and the normal group were given normal saline 25 mL/(kg·d)by gavage for 4 weeks,and those in the Gandou Decoction group were given Gandou Decoction 18.5 g/(kg·d)by gavage for 4 weeks.Inductively coupled plasma mass spectrometry was used to measure the content of copper in the liver;a biochemical analyzer was used to measure the serum levels of serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),alanine aminotransferase,and aspartate aminotransferase;the content of malondialdehyde(MDA)in liver tissue was measured to evaluate the degree of lipid peroxidation in the liver;oil red O staining was used to observe liver lipid deposition;RT-qPCR was used to measure the mRNA expression levels of lipid metabolism-related proteins[adipocyte TG hydrolase(ATGL),fatty acid synthase(FASN),acetyl-CoA carboxylase 1(ACC1),and sterol regulatory element-binding protein-1c(SREBP-1c)],peroxisome proliferator-activated receptor gamma(PPARγ),and cluster of differentiation 36(CD36)in liver tissue,and Western blot was used to measure the protein expression levels of PPARγand CD36 in the liver.Results Compared with the normal group,the model group had a significant increase in the content of copper in the liver(P<0.05),and compared with the model group,the Gandou Decoction group had a significant reduction in the content of copper in the liver(P<0.05).Compared with the normal group,the model group had significant reductions in the serum levels of TG,TC,and LDL-C(P<0.05),and compared with the model group,the Gandou Decoction group had significant increases in the serum levels of TG,TC,and LDL-C(P<0.05).The model group had a significant increase in the content of MDA in the li
作者
文佩华
汪世靖
董健健
徐陈陈
张培
徐乐文
程楠
WEN Peihua;WANG Shijing;DONG Jianjian;XU Chenchen;ZHANG Pei;XU Lewen;CHENG Nan(Institute of Neurology,Anhui University of Chinese Medicine,Anhui Hefei 230038,China)
出处
《安徽中医药大学学报》
CAS
2024年第6期80-85,共6页
Journal of Anhui University of Chinese Medicine
基金
安徽高校自然科学研究重点项目(KJ2021A0552)
安徽省自然科学基金项目(2208085MH226)。
关键词
肝豆状核变性
脂代谢
肝豆汤
PPARΓ
CD36
Wilson’s disease
Lipid metabolism
Gandou Decoction
Peroxisome proliferator-activated receptor gamma
Cluster of differentiation 36
