摘要
选择西藏湖泊巴松错为研究对象,利用16S rDNA Illumina高通量测序技术,研究巴松错水体不同样点、不同水域细菌群落组成和结构差异,探究环境因子与巴松错水体群落之间的相关关系。通过16S rDNA基因序列分析,共得到5570条有效OTU,注释获得88个门、180个纲、349个目、481个科和694个属。结果表明:变形菌门(Proteobacteria)、拟杆菌门(Bacteroidota)、放线菌门(Actinobacteria)是优势门类群;球形红杆菌属(Candidatus_Aquirestis)、hgcI_clade、unidentified_MethylacidipHilaceae是优势属类群;冗余分析显示,温度、溶解氧和生化需氧量是巴松错水体的细菌群落组成的重要环境因子;分子生态网络分析表明,变形菌门、放线菌门和拟杆菌门是网络构建的关键节点,94.5%的边为正相关关系。综上所述,巴松错水体细菌资源丰富,多样性较高,与环境因子关系密切,巴松错细菌群落间相互作用以协同作用为主导。研究为巴松错细菌资源多样性开发利用提供了理论基础。
In this study,we selected the Basomtso Lake in Xizang,China,as the research object,and utilized 16S rDNA Illumina high-throughput sequencing technology to investigate differences in the composition and structure of bacterial community across different locations and waters within Basomtso Lake.Our aim was to explore the correlation between environmental factors and the bacterial community in Basomtso Lake.Analysis of 16S rDNA gene sequences yielded a total of 5570 valid OTUs.with annotation revealing 88 phyla,180 classes,349 orders,481 families,and 694 genera.The results showed that Proteobacteria,Bacteroidota,and Actinobacteria were the dominant phyla,while Candidatus_Aquirestis,hgcI_clade,and unidentified_MethylacidipHilaceae were the dominant genera.Redundancy analyses showed that temperature,dissolved oxygen,and biochemical oxygen demand exerted significant effects on changes in the bacterial community structure in the water bodies of the Basomtso Lake.Molecular ecological network analysis showed that Ascomycetes,Actinobacteria,and Bacteroidetes as key nodes in the network construction,with 94.5%of edges displaying positive correlations.In summary,the bacterial resources in the Basomtso Lake are rich and diversified,closely related to environmental factors.Interactions among bacterial communities in Basomtso Lake are predominatly synergistic.This study provides an important theoretical foundation for the development and utilization of bacterial diversity in Basomtso Lake.
作者
周迪
王东旭
欧美香
格桑曲珍
德吉
刘琳
郭小芳
ZHOU Di;WANG Dong-Xu;OU Mei-Xiang;GESANG Qu-Zhen;DE Ji;LIU Lin;GUO Xiao-Fang(Institute of Ecology and Environment of Tibet University,Lhasa 850000,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2024年第12期2055-2068,共14页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(31960024)
西藏大学人才激励计划资助。
关键词
高原湖泊
细菌群落结构
环境因子
分子生态网络
协同作用
巴松错
Plateau lake
Bacterial community structure
Environmental factor
Molecular ecological network
Cooperative interaction
Basomtso Lake
