摘要
为探究红景天苷(Salidroside,SAL)是否通过抑制PI3K/AKT调控急性淋巴细胞白血病细胞生物学行为。本研究将稳定培养的急性淋巴细胞白血病Jurkat细胞分为对照组,SAL低剂量组,SAL中剂量组和SAL高剂量组。采用MTT法测定各组细胞增殖能力,采用流式细胞术检测细胞凋亡水平,采用Transwell试验检测细胞侵袭和迁移能力,采用Western blot检测各组细胞PI3K、AKT和p-AKT蛋白的表达水平。MTT结果显示,各组Jurkat细胞的细胞生长抑制率均随时间的延长而增加,且各时间点Jurkat细胞的细胞生长抑制率均随SAL剂量的增加而增加;流式细胞术结果表明,SAL可以提高Jurkat细胞的凋亡率,且随着SAL浓度的增加Jurkat细胞凋亡的凋亡率逐渐升高;Transwell结果显示,SAL可以抑制Jurkat细胞的侵袭和迁移能力,且随着SAL浓度的增加Jurkat细胞侵袭和迁移能力逐渐下降;Western blot检测结果显示,SAL处理后各组细胞中PI3K和p-AKT蛋白的表达水平均显著下调,p-AKT/AKT比值显著下降。SAL是一种抑制ALL细胞增殖、迁移和侵袭,并促进ALL细胞凋亡的新型治疗化合物。SAL的抗癌作用可能是通过调控PI3K-AKT信号通路实现的。
To investigate whether salidroside regulate the biological behaviour of acute lymphoblastic leukaemia cells through inhibition of PI3K/AKT.This study stably cultured acute lymphoblastic leukaemia Jurkat cells were divided into control,SAL low dose,SAL medium dose and SAL high dose groups.MTT assay was used to determine the proliferation ability of cells in each group,flowcytometry was used to detect the apoptosis level,transwell assay was used to detect the invasion and migration ability of cells,and western blot was used to detect the expression level of PI3K,AKT,and p-AKTproteins in cells in each group.MTTresults showed that the cell growth inhibition rate of Jurkat cells in all groups increased with time,and the cell growth inhibition rate of Jurkat cells at all time points increased with the increase of SAL dose;flowcytometry results showed that SALcould increase the apoptotic rate of Jurkat cells,and with the increase of SALconcentration Jurkat cell apoptotic apoptosis rate gradually increased;transwell results showed that SAL could inhibit the invasive and migratory ability of Jurkat cells,and the invasive and migratory ability of Jurkat cells gradually decreased with the increase of SAL concentration;results of western blot assay showed that the expression levels of PI3Kand p-AKTproteinswere significantly down-regulated in all groups of cells after SALtreatment SAL is a novel therapeutic compound that inhibits the proliferation,migration and invasion of ALL cells and promotes the apoptosis of ALL cells.The anticancer effect of SAL may be achieved through the modulation of the PI3K-AKT signalling pathway.
作者
赵洪波
李焱
刘亚宁
任欣欣
申智超
ZHAO Hongbo;LI Yan;LIUYaning;REN Xinxin;SHEN Zhichao(Department of Haematology,The First Hospital of Handan,Handan 056001,China)
出处
《特产研究》
2024年第6期47-52,共6页
Special Wild Economic Animal and Plant Research
基金
河北省邯郸市科学技术研究与发展计划项目(202130802020016)。
