摘要
目的:基于网络药理学方法探讨蒲黄治疗糖尿病视网膜病变(diabetic retinopathy,DR)的作用机制。方法:运用中药系统药理分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)提取蒲黄的活性成分及对应靶点,借助UniProt数据库对靶点进行规范化,通过GeneCards数据库和OMIM数据库获取DR的相关靶点,并利用R语言筛选药物与疾病的交集靶点。通过Cytoscape软件构建蒲黄“活性成分-疾病靶点”可视化调控网络,利用STRING数据库构建蛋白互相作用网络,并借助Bioconductor平台进行基因本体论(gene ontology,GO)富集分析及京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)富集通路分析。结果:共获得蒲黄6个活性成分,药物与疾病127个交集靶点,核心靶点包含蛋白激酶B、血管内皮生长因子A、肿瘤蛋白53等;GO功能富集分析涉及转录因子活性、酶活性、受体活性及生物化学过程调控等过程,KEGG通路富集分析涉及动脉粥样硬化、AGEs-RAGE等通路。结论:蒲黄中的槲皮素等活性成分作用于Akt1等疾病靶点,参与酶结合、应激反应等生物学功能调控AGEs-RAGE等信号通路,从而治疗DR。
Objective:To investigate the mechanism of treating diabetic retinopathy(DR)with Puhuang(typhae pollen)based on network pharmacology.Methods:TCMSP was used to extract the active ingredients and the corresponding targets of Puhuang,the standardization of the targets was conducted via Uniprot database,DR-related targets were obtained from GeneCards database and OMIM database,the intersecting targets between medicine and disease were screened using R language;visualization of"active ingredients-disease targets"control network of Puhuang was constructed via Cytoscape software,protein interaction network was built using STRING database,GO enrichment analysis and KEGG enrichment pathway was performed via Bioconductor platform.Results:The study has yielded six active ingredients,and 127 intersecting targets between the medicine and disease,core targets contained protein kinase B,vascular endothelial growth factor A,tumour protein 53 and others;GO functional enrichment analysis involve processes such as the activity of transcription factor,enzyme and receptor,and regulation of biochemical processes,and KEGG pathway enrichment analyses involve pathways such as atherosclerosis,AGEs-RAGE,etc.Conclusion:Active ingredients such as quercetin in Puhuang could act on the disease targets such as Akt1 and participate in biological functions including enzyme binding,and stress response,to regulate AGEs-RAGE signaling pathway,thereby treating DR.
作者
刘雅玲
唐勇华
黄慕槿
农吉晓
LIU Yaling;TANG Yonghua;HUANG Mujin;NONG Jixiao(Graduate School of Guangxi University of Chinese Medicine,Nanning 530011,China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530001,China)
出处
《西部中医药》
2024年第11期44-49,共6页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81860870)。
