摘要
目的:研究酒精性骨质疏松(AOP)大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的变化及中药生髓健骨胶囊对其影响,探讨生髓健骨胶囊对酒精性骨质疏松症大鼠模型的中药防治作用机理。方法:选取120只SD大鼠,随机分成4组(每组30只),分别命名为空白对照组、模型组、西药对照组和中药干预组。除空白对照组外,其他3组通过白酒灌胃法建立酒精性骨质疏松模型。西药对照组给予碳酸钙片与阿法D3混悬液灌胃治疗,中药干预组给予生髓健骨胶囊与0.9%生理盐水混悬液灌胃治疗。于造模第8,12,16周末取材,通过分光光度法测定大鼠血清中超氧化物歧化酶和丙二醛的含量。结果:检测造模干预8,12,16周后超氧化物歧化酶及丙二醛指标变化,模型组血清中超氧化物歧化酶含量低于空白对照组,丙二醛含量高于空白对照组,且差异有统计学意义(P<0.01);西药对照组和中药干预组的血清超氧化物歧化酶水平高于模型组,而丙二醛含量则低于模型组,差异有统计学意义(P<0.05),表明两组干预均能提高超氧化物歧化酶活性并降低丙二醛水平;中药干预组在预防酒精性骨质疏松方面的效果优于西药干预组,差异有统计学意义(P<0.05)。结论:血清中超氧化物歧化酶及丙二醛参与了大鼠酒精性骨质疏松症的病理进程,而生髓健骨胶囊可以调节酒精性骨质疏松症大鼠血清中超氧化物歧化酶及丙二醛含量,这是预防骨质疏松症的机制之一。
Objective:To study on the changes of superoxide dismutase(SOD)and malondialdehyde(MDA)in the serum of rats with alcoholic osteoporosis(AOP)and the effects of the traditional Chinese medicine Shengsui Jiangu capsules on them,and to explore the mechanism of the therapeutic efficacy of Shengsui Jiangu capsules on the AOP rat model.Methods:120 SD rats were selected,and were divided into four groups,each consisting of 30 rats.The groups were named as follows:blank control group,model group,Western medicine control group,and Chinese medicine intervention group.Except for the blank control group,the alcohol-induced osteoporosis models were established in other three groups by gavage with white liquor.After the modeling,the Western medicine control group was treated with a suspension made from calcium carbonate tablets and Alfa D3(a form of vitamin D3);the Chinese medicine intervention group was treated with a suspension made from Shengsui Jiangu capsules and 0.9%saline solution.At the end of weeks 8,12,and 16 post-modeling,samples were taken,and the levels of SOD and MDA in the rat serum were determined using spectrophotometry.Results:The study monitored the variations in SOD and MDA indicators after 8,12,and 16 weeks of intervention.The content of SOD in serum of model group was lower than that of blank control group,and the content of MDA was higher than that of blank control group,and the difference was statistically significant(P<0.01).In terms of serum SOD levels,both the Western medicine control group and the Chinese medicine intervention group had higher levels than that of the model group,and the MDA levels of the Western medicine control group and the Chinese medicine intervention group were lower than that of the model group,with statistically significant differences(P<0.05).It showed that both group interventions increased SOD activity and decreased MDA levels.Additionally,the Chinese medicine intervention group showed a more pronounced effect in preventing alcohol-induced osteoporosis than that of the Wester
作者
郑宇
谭福柱
任树军
ZHENG Yu;TAN Fuzhu;REN Shujun(Graduate School of Heilongjiang University of Chinese Medicine,Harbin 150040,China;First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中国中医骨伤科杂志》
CAS
2024年第11期1-4,10,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
黑龙江省自然科学基金联合引导项目(LH2020H086)
黑龙江省中医药管理局项目(ZHY2022-142)。
