摘要
CCT基因是指编码蛋白包含CCT(CONSTANS,CONSTANS-LIKE和TOC1)结构域的基因的统称,广泛存在于高等植物中,并且是与植物开花性状相关的重要基因家族之一,这些基因不仅参与了植物对光周期的响应过程,还在非生物胁迫应答以及植物激素信号转导等环节中发挥着重要作用。本研究利用生物信息学和比较基因组学的方法,最终在白菜型油菜(Brassica chinensis var.oleifera Makino et Nemoto)中鉴定到69个CCT家族基因,并对其理化性质、系统进化关系、保守结构域、基因结构、共线性关系、顺式作用元件、KEGG富集和不同组织/器官中的表达模式等进行了分析。结果表明,69个BrCCTs基因不均匀地分布在10条染色体上,根据保守结构域的不同,可以分为4个亚族,同一亚族成员的保守基序高度相似,然而基因结构差异较大,并且多数成员均含有光响应元件和激素响应元件,表明其可能在调节植物开花以及生长发育过程中发挥重要作用。KEGG富集分析发现CCT家族基因主要富集在细胞功能和核酸结合位点通路上。转录组结果表明,69个BrCCTs在根、茎、叶、花和愈伤组织中有着不同的表达模式,尤其COL亚族的成员表达水平较为显著。本研究为进一步了解白菜型油菜CCT基因家族的功能和作用提供了参考,为油菜早熟品种的选育和改良奠定了基础。
The term“CCT genes”refer to the genes encoding proteins containing CCT(CONSTANS,CONSTANS-LIKE and TOC1)domains,widely found in higher plants,and they are a pivotal gene family linked to flowering traits in plants.In-depth investigations have revealed that these genes not only participate in the photoperiodic response of plants but also play significant roles in abiotic stress responses and plant hormone signal transduction.Our study successfully identified 69 CCT family genes in Brassica rapa using bioinformatics and comparative genomics techniques and subsequently investigated their physicochemical properties,phylogenetic relationships,conserved domains,gene structures,syntenic relationships,cis-acting elements,KEGG enrichment,and expression patterns across diverse tissues/organs.Our findings revealed that the 69 BrCCTs genes are non-uniformly distributed across 10 chromosomes.We categorized them into four distinct subfamilies based on their conserved domains.Members belonging to the same subfamily exhibit remarkable similarity in their conserved motifs but display considerable differences in their gene structures.Notably,the majority of these genes harbor light-responsive and hormone-responsive elements,which is an indication of their potential roles in regulating plant flowering and growth development.KEGG enrichment analysis revealed that these genes are primarily enriched in cellular functions and nucleic acid binding sites.Additionally,transcriptome analysis showed the distinct expression patterns of the 69 BrCCTs in roots,stems,leaves,flowers,and callus tissues.Members of the COL subfamily displayed particularly prominent expression levels.Overall,this study offers profound insights into the functionalities and roles of the CCT gene family in B.rapa and thus provides a solid foundation for the selection and improvement of early-maturing B.rapa varieties.
作者
李华欣
王龙
马茜茹
裴大妹
肖麓
LI Huaxin;WANG Long;MA Qianru;PEI Damei;XIAO Lu(Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,Qinghai,China)
出处
《草业科学》
CAS
CSCD
北大核心
2024年第11期2637-2650,共14页
Pratacultural Science
基金
青海省“帅才科学家负责制”课题计划项目(2022-NK-170)
国家现代农业产业技术体系项目(CARS-12)
青海省自然科学基金项目(2022-ZJ-902)。
