摘要
试验以BPSE为鸭精液稀释液,探讨细管法冷冻、保存及解冻技术对鸭精液冷冻、解冻后活力、活率及受精率等的影响。比较不同体积分数的甘油(GL)、二甲基亚砜(DMSO)、二甲基乙酰胺(DMA)3种冷冻保护剂在4℃平衡不同时间对鸭精液活力的影响以及不同解冻条件和不同保存时间(1、30、365 d)对鸭精液活力和活率的影响,且评价冷冻后鸭精液与新鲜鸭精液(CK)的受精率和孵化率差异。结果表明,在6%DMA中平衡45 min和8%DMA中平衡30 min冷冻,解冻后的鸭精子活力较好,分别为0.416和0.415,二者间无显著差异;采用37℃30 s解冻后的鸭精液活率为57.18%,显著高于4℃3 min解冻后的精液活率(45.28%);在液氮中保存1、30、365 d,解冻后鸭精液的活力和活率差异均不显著;冷冻精液解冻后的受精率为37.73%,显著低于对照组的受精率(93.24%);冷冻鸭精液解冻后的孵化率为84.79%,显著低于对照组的孵化率(92.19%)。研究建立的在8%DMA中平衡30 min的细管法可用于鸭精液的冷冻保存,37℃解冻30 s可用于鸭精液的解冻。
In this study,BPSE was used as ducks semen diluent to explore the effect of thin tube freezing,preserving,and thawing technology on duck semen vitality,survival rate,and fertilization rate after cryopreservation and thawing.The effects of different volume fraction of 3 cryoprotectants including glycerol(GL),dimethyl sulfoxide(DMSO),and dimethyl acetamide(DMA)cryoprotectants at 4℃for different equilibrium times on duck semen vitality were compared,and the effects of different thawing condtions and storage time on semen vitality and survival rate were compared.The fertilization rate and hatching rate of frozen duck semen and fresh duck semen(CK)were evaluated.The results showed that the vitality of the duck semen after thawing was better when balanced for 45 min in 6%DMA and 30 min in 8%DMA,with values of 0.416 and 0.415,respectively,and there was no significant difference between the two group.The semen survival rate after thawing at 37℃for 30 s was 57.18%,significantly higher than that(45.28%)after thawing at 4℃for 3 min.There was no significant difference in semen vitality and survival rate after thawing in liquid nitrogen for 1,30,365 d;the fertilization rate of thawed frozen semen was 37%,which was significantly lower than that(93.24%)of the control group.The hatching rate of thawed frozen semen was 84.79%,which was significantly lower than that(92.19%)of the control group.The thin tube method established in the study balanced for 30 min in 8%DMA could be used for cryopreservation of duck semen,and thawing for 30 s at 37℃could be used for duck semen thawing.
作者
陶志云
宋卫涛
徐文娟
朱春红
刘宏祥
王逸飞
王志成
章双杰
李慧芳
TAO Zhiyun;SONG Weitao;XU Wenjuan;ZHU Chunhong;LIU Hongxiang;WANG Yifei;WANG Zhicheng;ZHANG Shuangjie;LI Huifang(Jiangsu Institute of Poultry Science,Yangzhou 225125,China)
出处
《山西农业科学》
2024年第6期122-127,共6页
Journal of Shanxi Agricultural Sciences
基金
“十四五”重点研发专项(2022YFD1300100)
扬州市科技计划项目(YZ2022050)
江苏省现代农业(水禽)产业技术体系(JATS[2023]365)
国家家养动物种质资源库(2024)。
关键词
鸭精液
细管法
冷冻保护剂
解冻温度
duck semen
thin tube method
cryoprotectant
thawing temperature
