摘要
为揭示可生物降解微塑料对不同沉水植物产生的生理生化影响,选择聚乳酸微塑料(PLA-MPs)为供试材料,以轮叶黑藻和苦草为供试沉水植物,深入探究不同暴露浓度下(5、20、50、100 mg·L^(-1))PLA-MPs对两种沉水植物株高、生物量、叶绿素含量和抗氧化酶活性方面的影响及其差异.结果表明:PLA-MPs对轮叶黑藻和苦草的鲜重、叶绿素a、叶绿素b以及总叶绿素含量存在抑制作用,且100 mg·L^(-1)PLA-MPs的抑制作用更显著.PLA-MPs显著影响两种沉水植物的抗氧化酶系统,两种沉水植物过氧化物酶(POD)活性和轮叶黑藻超氧化物歧化酶(SOD)活性在5 mg·L^(-1)PLA-MPs胁迫下表现出激活效应;当浓度增加到20 mg·L^(-1)时二者抗氧化酶活性开始降低.PLA-MPs在100 mg·L^(-1)浓度下对两种植物产生的生理生化影响最显著.苦草抗氧化系统对高浓度PLA-MPs的响应能力强于轮叶黑藻,推测苦草更能适应PLA-MPs污染水域.
In order to investigate effects of biodegradable microplastics on the physiological and biochemical functions of different submerged plants,polylactic acid microplastics(PLA-MPs)were used as the test material,with different concentrations(5,20,50,and 100 mg·L^(-1)),on the height,biomass,chlorophyll content,and antioxidant enzyme activities of two kinds of submerged plants,Hydrilla verticillata and Vallisneria natans.The results showed that PLA-MPs had an inhibitory impact on fresh weight,chlorophyll a,chlorophyll b,and total chlorophyll content of H.verticillata and V.natans,with 100 mg·L^(-1)PLA-MPs concentration showed more significant effect.The presence of PLA-MPs significantly affected the antioxidant enzyme systems of the two submerged plants.The peroxidase(POD)activities of both submerged plants and the superoxide(SOD)activities of H.verticillata showed activation under the stress of 5 mg·L^(-1)PLA-MPs.However,an increase in the concentration to 20 mg·L^(-1)decrease in the antioxidant enzyme activities.PLA-MPs produced the most substantial physiological and biochemical influences on both plants at concentrate of 100 mg·L^(-1).In comparison,the response ability of V.natans antioxidant system to the high concentration of PLA-MPs was stronger than that of H.verticillata.It means that V.natans is more adaptable to PLA-MPs-polluted waters.
作者
陆灿
姜翠玲
冯亚坤
蒋雷
孙磊
LU Can;JIANG Cuiling;FENG Yakun;JIANG Lei;SUN Lei(College of Hydrology and Water Resources,Hohai University,Nanjing 210098)
出处
《环境科学学报》
CAS
CSCD
北大核心
2024年第11期378-385,共8页
Acta Scientiae Circumstantiae
基金
国家重点研发计划(No.2023YFC3207500)。
关键词
微塑料
沉水植物
生理响应
叶绿素
抗氧化酶
microplastics
submerged plant
physiology response
chlorophyll
antioxidant enzyme
