摘要
为明确无瓣海桑炭疽病的病原菌种类,为该病害的有效防控提供基本依据,采集无瓣海桑Sonneratia apetala受害叶片,通过组织分离法获得菌株,利用柯赫氏法则确定病原菌;利用形态学及分子生物学分析方法明确病原菌的分类地位,同时开展生物学特性分析。通过接种试验确定,从病组织中分离获得菌株WBHS-YK-1为无瓣海桑炭疽病的病原菌。该菌在PDA上培养7 d,菌落圆形,气生菌丝白色,培养基底部有青黑色色素沉淀,同时产生黄色的分生孢子堆;分生孢子呈长椭圆,无色,无隔膜,孢子大小为(15~19)μm×(6~8)μm。基于ITS序列构建的系统发育树显示:该菌株与菌种Colletotrichum fructicola聚在同一分支,支持率高达98%,表明该菌与C.fructicola具有较高的亲缘关系。结合形态学特征,最终确定菌株WBHS-YK-1为果生炭疽菌C.fructicola。该菌能最佳碳源为葡萄糖,最佳氮源为蛋白胨;最适培养温度为30℃,最适pH为7~8。
The pathogen was identified to provide a theoretical basis for effective prevention and control of anthracnose in Sonneratia apetala.Microbes were isolated and purified from the S.apetala leaves showing anthracnose symptoms.Using Koch′s method,the pathogenicity of the isolates was tested and verified.Thetaxonomy of pathogens was determined by morphological observations and molecular technology.Strain WBHSYK-1 was isolated from the diseased tissue and proved to be the pathogen that caused the anthracnose symptoms in S.apetala leaves.The colonies WBHS-YK-1 were white with cyan-black precipitates round after 7 d on PDA.The conidia were elliptical and 15-19μm×6-8μm in size.ITS fragments were used to construct the phylogenetic tree that showed WBHS-YK-1 to cluster with Colletotrichum fructicola in the same branch,the support rate was as high as 98%.The best carbon source for this bacterium is glucose,and the best nitrogen source is peptone;The optimal cultivation temperature is 30℃and the optimal pH is 7-8.
作者
肖海燕
冯金艳
陆建康
田龙艳
张良军
郑世敬
徐金柱
肖丽娜
XIAO Haiyan;FENG Jinyan;LU Jiankang;TIAN Longyan;ZHANG Liangjun;ZHENG Shijing;XU Jinzhu;XIAO Lina(State-owned Forest Farm Management Office of Shaoguan City,Shaoguan,Guangdong 512000,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China)
出处
《林业与环境科学》
2024年第5期39-45,共7页
Forestry and Environmental Science
基金
广东省林业科技创新项目(2023KJCX020)。
关键词
无瓣海桑炭疽病
果生刺盘孢
生物学特性
anthracnose of Sonneratia apetala
Colletotrichum fructicola
biological characteristics
