摘要
外泌体是多泡体与细胞质膜融合时释放的细胞外囊泡,含有蛋白质、脂质和核酸等.它以细胞外囊泡的形式运送货物,参与多种癌症过程(如侵袭和转移),被认为是液体活检的新兴靶标,其在细胞通讯、信号传导和免疫应答中发挥着重要的作用.质谱法已成为蛋白质组学研究领域不可或缺的一部分,外泌体的蛋白质组学分析是发现潜在癌症生物标志物的一种很有前途的方法.高分辨率分离、高效质谱分析和全蛋白质组数据库的最新进展都有助于患者样本中外泌体的成功分析.本文综合评述了外泌体的分离方法、蛋白质组学分析技术以及基于外泌体的蛋白质组学分析在临床疾病诊断的应用研究.最后,对外泌体分离和蛋白质组学面临的挑战及在临床应用中的前景进行了展望.
Exosomes are extracellular vesicles released via the fusion of multivesicular bodies with the cell plasma membrane,containing proteins,lipids,nucleic acids,etc.They transport cargo in the form of extracellular vesicles and participate in various cancer processes such as invasion and metastasis.As emerging targets for liquid biopsy,exosomes play crucial roles in cell communication,signal transduction,and immune response.Mass spectrometry has become an indispensable part of the field of proteomics research,and the proteomic analysis of exosomes is a promising method for discovering potential cancer biomarkers.Recent advances in high-resolution separations,high-performance mass spectrometry and comprehensive proteome databases have all contributed to the successful analysis of exosomes from patient samples.Here,this article reviews the isolation methods of exosomes,proteomics analysis techniques,and the application research of proteomics analysis based on exosomes in clinical disease diagnosis.Finally,the challenges faced by exosome isolation and proteomics,as well as the prospects for their clinical applications,are discussed and outlooked.
作者
靳莹
张俊杰
张毅欣
袁悦
韩珍珍
JIN Ying;ZHANG Junjie;ZHANG Yixin;YUAN Yue;HAN Zhenzhen(School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China;Department of Pediatric Orthopedics,Anhui Children’s Hospital(Children’s Hospital of Anhui Medical University),Hefei 230051,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2024年第11期28-47,共20页
Chemical Journal of Chinese Universities
基金
安徽省高校自然科学研究项目(批准号:2023AH050572)
安徽省转化医学研究院科研基金(批准号:2023zhyx-C02)资助。
