摘要
以贵长猕猴桃茎段为材料,在基本培养基(MS)上诱导其产生愈伤组织,采用响应面法优化贵长猕猴桃愈伤组织诱导条件。在单因素分析的基础上,以6-苄基腺嘌呤(6-BA)质量浓度、2, 4-二氯苯氧乙酸(2,4-D)质量浓度和0.1%氯化汞(HgCl_(2))处理外植体时间为自变量,以贵长猕猴桃茎段愈伤组织的诱导率为评价指标,设计三因素三水平响应面法优化实验。结果表明,贵长猕猴桃最优愈伤组织诱导条件为:6-BA质量浓度为0.4 mg·L^(-1)、2, 4-D质量浓度为2.0 mg·L^(-1)、0.1%氯化汞处理时间为4.0 min,该诱导条件下贵长猕猴桃茎段愈伤组织诱导率为85.71%,与预测值的相对误差较小,实验值与预测值较吻合,贵长猕猴桃茎段愈伤组织诱导条件经优化后诱导率得到有效提高,可为贵长猕猴桃组织培养的进一步研究提供依据。
Using the stem segments of kiwifruit as materials,callus induction was induced on MS medium,and the response surface methodology was used to optimize the induction conditions of kiwifruit callus.On the basis of single factor analysis,a three factor three-level response surface methodology was designed to optimize the experiment,with 6-BA mass concentration,2,4-D mass concentration,and 0.1%mercuric chloride treatment time as independent variables,and the induction rate of callus tissue in the stem segment of kiwifruit as the evaluation index.The results showed that the optimal conditions for inducing callus tissue in kiwifruit were 6-BA concentration of 0.4 mg·L^(-1),2,4-D concentration of 2.0 mg·L^(-1),and 0.1%mercuric chloride treatment time of 4.0 minutes.Under these induction conditions,the induction rate of callus tissue in kiwifruit stem segments was 85.71%,which had a relatively small relative error with the predicted value.The experimental value was in good agreement with the predicted value.After optimizing the induction conditions,the induction rate of callus tissue in kiwifruit stem segments was effectively improved,providing a basis for further research on kiwifruit tissue culture.
作者
杨椿玉
辛佳乐
潘小舆
班虎检
韩世明
王月霞
YANG Chunyu;XIN Jiale;PAN Xiaoyu;BAN Hujian;HAN Shiming;WANG Yuexia(School of Biological Science and Technology,Liupanshui Normal University,Liupanshui 553000,Guizhou)
出处
《浙江农业科学》
2024年第11期2601-2606,共6页
Journal of Zhejiang Agricultural Sciences
基金
贵州省高等学校猕猴桃资源开发与利用重点实验室(黔教技[2022]054)
河南省果树瓜类生物学重点实验室(HNS-201907-03)
六盘水师范学院项目(LPSSYKYJJ201601,生物科学LPSSYYLZY2003,LPSSYZXXM202304,LPSSY2023XKTD09)
六盘水师范学院大学生创新创业训练计划项目(2021cxcy97)
六盘水市科技局项目(52020-2020-0906)。
关键词
贵长猕猴桃
愈伤组织
茎段
组织培养
响应面法
Guichang kiwifruit
callus
stem segments
tissue culture
response surface methodology
