摘要
利用傅里叶变换红外光谱(FTIR)结合二阶导数红外光谱,对5个不同产地的金环胡蜂蜂毒进行分析和鉴定。结果表明,不同产地的金环胡蜂蜂毒的一维红外光谱在1 659~1 658 cm^(-1)、1 545~1 539 cm^(-1)、1 467~1 455 cm^(-1)、1 100~1 041 cm^(-1)等处都有表征蛋白质、氨基酸、多糖、生物碱以及芳香类化合物等成分的特征吸收。但因产地不同,各样本吸收峰的位置和强度均存在一定差异。此外,1 467~1 455 cm^(-1)、1 386~1 368 cm^(-1)和1 100~1 041 cm^(-1)范围处的吸收峰是识别不同蜂毒样本主要特征峰。比较各产地金环胡蜂蜂毒的二阶导数红外光谱发现,1 512、1 500 cm^(-1)附近处的吸收峰进一步确认金环胡蜂蜂毒中的芳香类化合物的存在。此外,各样本在1 712 cm^(-1)和1 500 cm^(-1)附近的吸收峰以及1 700~1 620 cm^(-1)范围的吸收峰强度和位置均有明显差异。应用红外光谱、二阶导数红外光谱,不仅可以提供金环胡蜂蜂毒主要化学成分的相关信息,还可以对不同产地的金环胡蜂蜂毒进行很好的区分。该方法快速、直观、简便,能够为不同产地的金环胡蜂蜂毒的整体化学成分提供大量信息,有助于金环胡蜂蜂毒的整体质量控制及有效成分定性分析研究。
Fourier transform infrared spectroscopy(FTIR)combined with second derivative infrared spectroscopy was used to analyze and identify the venom of five different origins of golden ring wasps.The results showed that the one-dimensional infrared spectra of wasp venom from different origins exhibited characteristic absorption of proteins,amino acids,polysaccharides,alkaloids,and aromatic compounds at 1659~1658 cm^(-1),1545~1539 cm^(-1),1467~1455 cm^(-1),1100~1041 cm^(-1),and other locations.However,due to different origins,there are certain differences in the position and intensity of the absorption peaks of each sample.In addition,the absorption peaks in the ranges of 1467~1455 cm^(-1),1386~1368 cm^(-1),and 1100~1041 cm^(-1) are the main characteristic peaks for identifying different bee venom samples.Comparing the second derivative infrared spectra of wasp venom from different regions,it was found that the absorption peaks near 1512 and 1500 cm^(-1) further confirmed the presence of aromatic compounds in wasp venom.In addition,there were significant differences in the intensity and position of the absorption peaks near 1712 cm^(-1) and 1500 cm^(-1),as well as the absorption peak in the range of 1700 to 1620 cm^(-1) among the samples.The application of infrared spectroscopy and second derivative infrared spectroscopy can not only provide relevant information on the main chemical components of the venom of the golden ring wasp,but also effectively distinguish the venom of the golden ring wasp from different origins.This method is fast,intuitive,and simple,and can provide a large amount of information on the overall chemical composition of wasp venom from different origins,which is helpful for the overall quality control and qualitative analysis of effective ingredients in wasp venom.
作者
杨兰芬
李兆云
夏雪芬
杨金燕
杨琦
Yang Lanfen;Li Zhaoyun;Xia Xuefen;Yang Jinyan;Yang Qi(College of Agriculture and Biological Sciences,Dehong Teachers'College,Mangshi Yunnan 678400,China;Dehong Biomedical Engineering Research Center,Mangshi Yunnan 678400,China)
出处
《山西化工》
CAS
2024年第10期42-45,共4页
Shanxi Chemical Industry
基金
云南省教育厅科学研究基金项目(2020J0837)
云南省科学技术厅云南中医药大学应用基础研究联合专项(202001AZ070001-074)
德宏州创新团队(2021RC0011)
德宏州中青年学术和技术带头人项目(2021RC002)。
关键词
金环胡蜂
蜂毒
产地
傅里叶变换红外光谱
golden ring wasp
bee venom
place of origin
fourier transform infrared spectroscopy
