摘要
文章研究低表达s FRP4通过PI3K/AKT信号通路对肝癌细胞的具体作用机制,从而指导肝癌防治。实验选取肝细胞L-02、肝癌Huh7、Hep3B、Hep G2和MHCC97-H细胞进行细胞培养,以检测肝癌细胞和肝细胞内sFRP4蛋白表达、sFRP4m RNA表达、sFRP4沉默后HepG2细胞的增殖活性、各组细胞内PI3K、AKT、p21、Bcl-2、c-Myc蛋白与m RNA的表达情况,并对各项检测数据进行统计学分析。研究发现,肝癌细胞内s FRP4的表达情况高于肝细胞;sFRP4沉默后肝癌细胞的增殖、侵袭、转移能力增强,细胞凋亡减少;sFRP4沉默后PI3K/AKT信号通路相关因子的表达明显增强;肝癌中存在PI3K、p-AKT的异常表达,PI3K、AKT信号通路激活会增强肝癌细胞的增殖性、侵袭性、转移性。结果表明,低表达sFRP4通过PI3K/AKT信号通路可以促进肝癌细胞的增殖和侵袭,降低细胞凋亡率。
The paper investigated the specific mechanism of low expression sFRP4 acting on liver cancer cells through the PI3K/AKT signaling pathway,in order to guide the prevention and treatment of liver cancer.Liver cell L-02,liver cancer cell Huh7,Hep3B,and HepG2,and MHCC97-H were selected and cultured.The expression of sFRP4 protein and sFRP4 mRNA in liver cancer cells and liver cells,the proliferation activity of HepG2 cells after sFRP4 silencing,and the expression levels of PI3K,AKT,p21,Bcl-2,c-Myc protein and mRNA in each group of cells were detected.The results of each detection were statistically analyzed.The research founded that the expression of sFRP4 in liver cancer cells was higher than that in liver cells;after sFRP4 silencing,the proliferation,invasion,and metastasis of liver cancer cells were inhibited,and cell apoptosis reduced;after sFRP4 silencing,the expression of PI3K/AKT signaling pathway related factors was significantly inhibited.It indicated that there was abnormal expression of PI3K and p-AKT in liver cancer,and activation of PI3K,and AKT signaling pathways can enhance the proliferation,invasion,and metastasis of liver cancer cells.The results showed that low expression sFRP4 promoted the proliferation and invasion of liver cancer cells and reduced cell apoptosis through the PI3K/AKT signaling pathway.
作者
蒋渝
JIANG Yu(Yueyang Vocational Technical College,Yueyang 414000,China)
出处
《工业微生物》
CAS
2024年第5期44-46,共3页
Industrial Microbiology
基金
湖南省教育厅科研项目“sFRP4通过PI3K/AKT信号通路作用于肝癌细胞的机制研究”(编号:22C1362)。
